|
|
|
Expr11290
|
Eeak expression, pharynx: pm6-8, mc1-3, some glands, pharyngo-intestinal valve, gut, rectal glands, body wall muscle (adult only), in young larvae: unidentified head cell (head mesodermal cell?), CAN. |
|
|
|
|
Expr14587
|
The integrated smoc-1 transcriptional reporter showed strong GFP expression. GFP was first detectable in several cells located in the anterior of bean stage embryos. In the developing larvae, GFP is expressed in cells of the pharynx, the intestine and the posterior hypodermis. Pharyngeal cells expressing smoc-1p::gfp include the epithelial cells e2, the marginal cells mc1 and mc2, the M4 neuron, and all six of the pharyngeal/intestinal valve cells. Cells of the posterior hypodermis expressing smoc-1p::gfp include hyp8, hyp9, hyp10, and hyp11. Expression in these tissues persisted from the L1 larval stage through adulthood. We noted that while all transgenic animals showed GFP expression in the pharynx and the posterior hypodermis, a small fraction of animals (~8%) did not exhibit GFP expression in all or some of the intestinal cells. We observed no GFP expression in any other tissues, including the nerve cord, body wall muscles (BWMs), or the M lineage. |
|
|
Picture: Fig 3. |
|
Expr8678
|
Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm1, pm2, pm3, pm6, pm7, pm8, mc1, mc2, mc3. Weak or rare expression in pm4, pm5. Expression in the nervous system: DDn, DVA, DVB, DVC, PVP. Expression in the reproductive system: In adult stage, expressed in proximal gonad sheath, spermatheca. In developing larva stage, expressed in uterus, spermatheca. inx-10 is localized to pharyngeal precursors from early stages of embryogenesis, and by three-fold stage, all pharyngeal muscles except pm4 are seen to express it at high levels. |
|
|
Picture: Fig 2A to 2G. |
|
Expr9051
|
In wild-type embryos, ten-1a::gfp is first expressed in a cluster of cells in the anterior half at approximately 150 minutes after fertilization. These cells are precursors to the hypodermal cells, which are evident at 300 minutes post-fertilization, when the cells intercalate and begin the process of ventral closure, and to pharyngeal and intestinal cells, which are evident beginning at the bean stage. In later stages, strong expression of ten-1a::gfp persists in pharyngeal and intestinal cells, and appears in several head neurons. Examination of L1 larvae and adults allowed us to identify 8 pharyngeal cells that express ten-1a::gfp: the three marginal cells mc1, the three marginal cells mc3, and the neurons M2L and M2R. Adults also express ten-1a::gfp in vulva muscles, the gonad distal tip cells, the intestine, several tail neurons including DVB and some other cells. |
|
|
|
|
Expr11259
|
pax-1::HIS-GFP is expressed in the nuclei of all three groups of marginal cells, pm8 and v1. |
|
|
Picture: Fig 6, Fig 7. |
|
Expr8860
|
SMF-2::GFP was mostly restricted to the mc1, mc2, mc3 epithelial cells of the pharynx and the pharyngeal-intestinal valve cells vpi1 to 6, displaying an anterior-posterior expression gradient. SMF-2 was also observed in the gonad sheath cells at the adult stage. The GFP signal was detected from late embryogenesis to adult stage, with generally higher expression levels in young larvae (L1 stage). |
SMF-2::GFP was associated with intracellular cytoplasmic compartments and to a lesser extent to the apical membrane of the mc epithelial cells. |
|
|
|
Expr11324
|
Fairly weak expression, pharynx: pm3,4,6,7,8 mc1,3, glands (strong), excretory cell, hypodermis (borderline detectable). |
|
|
|
|
Expr2624
|
Promoter-GFP transgenes indicated that lam-3 is expressed during gastrulation and through embryogenesis in pharyngeal, intestinal and epidermal cells. In the larvae, lam-3 gene expression is maintained in the spermatheca and in the pharyngeal m3-m8 and mc cells. |
|
|
|
|
Expr16520
|
Ppa-nas-6 was expressed in the pharyngeal muscles (pm2), arcade cells, epithelial cells (e1, e2, e3), marginal cells, (mc1, mc2, mc3), amphid neurones, and the pharyngeal-intestinal valve. |
|
|
Picture: Fig 3. |
|
Expr8677
|
Expression in the alimentary canal: Strong and consistent expression in anterior arcades, posterior arcades. Weak or rare expression in pm2, pm3, pm4, pm5, pm6, pm7, pm8, mc1, g1, g2, rectal gland cells, rectal epithelial cells. Expression in the nervous system: Phsh, AVK, DVC (early larva), PVR, SIB (early larva), URB, I3. Expression in the reproductive system: In adult stage, expressed in gonad sheath, uterus, vulval muscle. In developing larva stage, expressed in vulva. Neuronal expression of inx-9 appears around three-fold stage. The rectal gland expresses inx-9 during early larval stages. inx-9 is expressed in adult hermaphrodite sex muscles. inx-9 was expressed at high levels in arcade cells starting around two-fold stage continuing throughout development and adulthood. |
|
|
|
|
Expr15821
|
We first used the gpa-16 promoter (Jansen et al., 1999) to broadly drive the expression of GCaMP3 in pharyngeal muscle. We characterized the expression pattern of this transgene using confocal microscopy and observed bright fluorescence in the pm2 and pm3 muscles and also in the mc1 marginal cells. We also observed occasional dim fluorescence in pm1 (3 of 10 animals). |
|
|
|
|
Expr15822
|
C32F10.8p::GFP was expressed in both the pm3s and mc1s but not in pm1 or the pm2s. |
|
|
Picture: N.A. |
|
Expr8691
|
Expression in the alimentary canal: Strong and consistent expression in pm2, pm4, pm5, mc1, mc2. Weak or rare expression in pm3, pm6, pm7, pm8, K.a/K$(B!G(B cells. |
|
|
|
|
Expr13074
|
All the invertebrate lysozymes have in common an intestinal expression pattern. However, one subset appeared to have additional pharyngeal expression whereas the other exhibited some neuronal promoter activity. ilys-1 expressed in the pm3 cells in the procorpus, the pm4 cells in the anterior bulb pharyngeal muscles (sieve) and the marginal cells mc1 and mc2. |
|
|
Other strain-- UL820 |
|
Expr154
|
Expression is seen in the marginal cells of the pharynx, from early larval stages until the adult stage. In early larvae the mc1 and mc2 marginal cells show expression, whereas in adults only the mc2 marginal cells are seen to express. |
|
|
|
|
Expr16580
|
We used a putative promoter 5 kb sequence immediately upstream of the start codon of pezo-1 isoforms i and j. GFP expression was mainly restricted to pharyngeal muscles and sensory neurons. In the pharynx, we saw GFP labeling of the pm3 muscle cells, the mc1 marginal cells in the corpus, a structure responsible for generating suction forces during the ingestion of suspended bacteria (Raizen et al. 2012), and in a neuron consistent in location and morphology with I4. A ventral cell located posterior to the terminal bulb and extending a process dorso-anteriorly toward the pharyngeal-intestinal valve (consistent with the excretory gland) was also labeled. Based on their location and characteristic extensive branching, we identified the anterior and posterior mechanoreceptors FLP and PVD (respectively) as also expressing GFP. Posteriorly, we tentatively identified a phasmid neuron consistent with the PHC neuron's morphology and location, as well as other cells associated with the uterus and anus, as expressing GFP. Unlike the other transcriptional constructs, GFP expression in these animals was visible as early as prezle-stage embryos. |
|
