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Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. |
DESeq. False discovry rate (FDR) < 0.1. |
WBPaper00048988:neuron_expressed
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Transcripts that showed significantly increased expression in ogt-1(ok1474) neuronal cells isolated by FACs comparing to in FACs isolated neuronal cells from wild type. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00066485:ogt-1(ok1474)_upregulated_neuron
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Bacteria infection: Enterococcus faecalis |
Genes with increased expression after 24 hours of infection by E.faecalis Fold changes shown are pathogen vs OP50. |
For RNA-seq and tiling arrays, log2 fold changes between gene expression values of infected versus uninfected nematodes were calculated. For log2 fold changes > 0.00001 the values > 81.25th percentile were defined as up-regulated and for log2 fold changes < -0.00001 the values < 18.75th percentile were defined as down-regulated. |
WBPaper00038438:E.faecalis_24hr_upregulated_TilingArray
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Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:Rifampicin_upregulated
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Transcripts that showed significantly increased expression in hsf-1(sy441) vs. in N2 day 1 adults without heat shock. |
edgeR, fold change > 2, FDR < 0.05 |
WBPaper00066900:hsf-1(sy441)_upregulated
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Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. |
Cufflinks FPKM value >=1. |
WBPaper00050990:intestine_expressed
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Transcripts that showed significantly increased expression after four-day-old young adult worms were placed on NGM plates seeded with OP50 in the presence 5% Agaro-oligosaccharides(AGO) for 24 h, comparing to animals grown in the absence of AGO. |
Fold change > 2. |
WBPaper00064306:Agaro-oligosaccharides_upregulated
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Transcripts that showed significantly decreased expression in vit-2(ac3); zcIs4, comparing to parenting strain SJ4005 [zcIs4]. |
Differential gene expression analysis was then performed on normalized samples. Genes exhibiting at least twofold change and a false-discovery rate (FDR) of 1% or less were considered differentially expressed. |
WBPaper00051305:vit-2(ac3)_downregulated
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Transcripts that showed significantly decreased expression in daf-16(mgDf50) comparing to in N2 at L1 larva stage. |
DESeq v1.20.0 was used to analyze differential gene expression. Transcripts with adjusted p-value < 0.05 were considered differentialled expressed. |
WBPaper00048971:daf-16(mgDf50)_downregulated_L1
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Transcripts that showed significantly increased expression in hda-1(ne4752[3xFLAG-Degron-HDA-1]) in gonads dissected from 1-day old adult animals. |
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R. |
WBPaper00061479:hda-1(ne4752)_upregulated
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Transcripts that showed significantly increased expression in mep-1(ne4629[MEP-1-GFP-Degron]) in gonads dissected from 1-day old adult animals. |
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R. |
WBPaper00061479:mep-1(ne4629)_upregulated
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Transcripts that showed significantly increased expression in mut-16(pk710), comparing to in N2 animals. |
DESeq2 v. 1.22.2, adjusted p-value <= 0.05. |
WBPaper00059605:mut-16(pk710)_upregulated
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Transcripts that showed significantly decreased expression in eat-2(ad1116) comparing to in N2 at 3-days post L4 adult hermaphrodite animals. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:eat-2(ad1116)_downregulated
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Transcripts that showed significantly increased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(+), comparing to in control animals, at 2-day post L4 adult hermaphrodite stage. |
DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. |
WBPaper00050859:upregulated_P-granule(-)GFP(+)_vs_control_day2-adult
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Transcripts that showed significantly increased expression in animals lacking P granules by RNAi experiments targeting pgl-1, pgl-3, glh-1 and glh-4, and unc-119-GFP(-), comparing to in control animals, at 2-day post L4 adult hermaphrodite stage. |
DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. |
WBPaper00050859:upregulated_P-granule(-)GFP(-)_vs_control_day2-adult
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Transcripts that showed significantly increased expression in pgl-1(ct131) animals (isolated from SS0002[pgl-1(ct131)him-3(e1147)], comparing to in control animals SS1174, at 1-day post L4 adult hermaphrodite stage. |
DESeq2, Benjamini-Hochberg multiple hypothesis corrected p-value < 0.05 and fold change > 2. |
WBPaper00050859:upregulated_pgl-1(ct131)_vs_control_day1-adult
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Transcripts that showed significantly increased expression in hsf-1(sy441) after worms were heat shocked at 35C for 30 minutes followed with a 1-hour long recovery at 20C, comparing to N2 animals with the same treatment. |
edgeR, fold change > 2, FDR < 0.05 |
WBPaper00066900:hsf-1(sy441)_upregulated_heat-shock
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Transcripts that showed siginificantly increased expression in nmad-1(ok3133) comparing to in N2 at 25 entigrade. |
edgeR, FDR < 0.05, fold change > 2. |
WBPaper00056997:nmad-1(ok3133)_upregulated_germline_25C
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Transcripts that showed significantly lower expression in somatic gonad precursor cells (SGP) vs. head mesodermal cells (hmc). |
DESeq2, fold change >= 2, FDR <= 0.01. |
WBPaper00056826:hmc_biased
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Transcripts that showed significantly increased expression in animals with pan-neuronal expression of human amyloid beta (snb-1p-Amyloid-beta(1-42) + mtl-2p-GFP), comparing to in N2 animals. |
edgeR p-value < 0.01 and fold change > 4. |
WBPaper00061613:human-amyloid-beta_upregulated
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Genes identified as up-regulated at a 5% false discovery rate through RNAseq experiments with three tatn-1(qd182) and three N2 RNA samples. |
ANOVA with FDR <= 0.05. |
WBPaper00044656:tatn-1(qd182)_upregulated
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Transcripts that showed significantly increased expression after animals were treated with 100uM Rapamycin, 50uM Rifampicin and 250uM Allantoin from day 1 to day 3 adult hermaphradite. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:Rapamycin-Rifampicin-Allantoin_upregulated
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Targets of endo-siRNA that showed significantly decreased expression in the whole animal of JH3225[meg-3(tm4259) meg-4(ax2026)] comparing to in N2. |
DESeq2 1.22.2, fold change > 2, FDR < 0.05 |
WBPaper00057161:meg-3(tm4259)_meg-4(ax2026)_downregulated_endo-siRNA_targets
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Multigenerational temperature shift. |
Transcripts that were induced in hrde-1(tm1200) animals by multigenerational heat stress. |
The Bio-conductor R package of DEseq was used for this analysis. Heat-induced genes were defined as genes with at least twofold increase in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats. Heat-repressed genes were defined as genes with at least two-fold decreases in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats. |
WBPaper00049098:heat-induced_hrde-1(tm1200)
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Transcriptions that showed significantly decreased expression in skn-1(RNAi) comparing to empty vector injection into rrf-3(pk1426);daf-2(e1368) animals. |
Genes with an absolute fold changeof at least 2 and standard p-values below 0.05 were considered as differentially expressed. |
WBPaper00062193:skn-1(RNAi)_downregulated
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Transcripts that showed significantly increased expression in hrde-1(tm1200) in gonads dissected from 1-day old adult animals. |
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R. |
WBPaper00061479:hrde-1(tm1200)_upregulated
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Transcripts that were induced by hrde-1(tm1200) at 23C comparing to in N2 animals. |
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1. |
WBPaper00049098:hrde-1(tm1200)-induced_23C
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Transcripts that showed significantly increased expression after animals were treated with 50mM Metformin from day 1 to day 3 adult hermaphradite. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:Metformin_upregulated
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Proteins that bind PRG-1 according to immunoprecipitation and mass spectrometry. |
To estimate the significance of the change in protein abundance, a linear model (adjusted for peptides and biological replicates) was performed, and P values were FDR-adjusted using the BenjaminiHochberg procedure with a control threshold set to 0.05. |
WBPaper00059254:PRG-1_interacting
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