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Genes that were not enriched in either spermatogenic fem-3(q96gf) nor oogenic fog-2(q71) gonads, according to RNAseq analysis. |
To identify differentially expressed transcripts, authors used R/Bioconductor package DESeq. |
WBPaper00045521:Gender_Neutral
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Transcripts that showed significantly increased expression in set-2(tm1630) animals at embryo stage, comparing to in N2 animals. |
DESeq2 (v2.1.8.3) was used to determine DE genes and to generate principal component and scatter plots. DE genes with FDR < 0.05 were analysed using g:Profiler with Bonferroni correction. |
WBPaper00060014:set-2(tm1630)_upregulated
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Temperature Shift: 25C vs 15C for 16 hours at L4 larva stage. |
Transcripts that showed significantly increased expression in AFD neurons comparing to in whole animals. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00065158:AFD_enriched
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Transcripts that showed significantly decreased expression in ogt-1(ok1474) neuronal cells isolated by FACs comparing to in FACs isolated neuronal cells from wild type. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00066485:ogt-1(ok1474)_downregulated_neuron
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ncRNAs with significantly increased expression in cep-1(gk138) than in N2 under normal conditions, according to RNAseq. |
ncRNAs with more than 2 fold difference in expression are considered differentially expressed |
WBPaper00045380:cep-1(gk138)_upregulated_normal
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Bacteria: C.neoformans strain H99 |
Transcripts that showed significantly increased expression after infection by C. neoformans H99. |
Ballgown was used to calculate differential expression of genes using FPKM data and to generate tables with fold change and P values. Genes were shortlisted with a cutoff of 2-fold change and P values of less than 0.05. |
WBPaper00059754:C.neoformans_H99_upregulated
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Bacteria infection: Pseudomonas aeruginosa PA14. 24 hours of exposure. |
Small RNAs (21-26nt) that showed significantly decreased expression after L4 animals were exposed to P .aeruginosa strain PA14 for 24 hours. |
DESeq2, FDR < 0.05 |
WBPaper00056868:P.aeruginosa_downregulated_smallRNA
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ncRNAs with significantly increased expression in cep-1(gk138) than in N2 after UV irradiation, according to RNAseq. |
ncRNAs with more than 2 fold difference in expression are considered differentially expressed |
WBPaper00045380:cep-1(gk138)_upregulated_UV
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