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Transcripts that showed significantly decreased expression in eat-2(ad1116) comparing to in N2 at 3-days post L4 adult hermaphrodite animals. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:eat-2(ad1116)_downregulated
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Transcripts that showed significantly decreased expression in N2 comparing to in skn-1 gain of function allele lax188 animals |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00067765:skn-1(lax188)_upregulated_N2
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Germline-enriched and sex-biased expression profile cluster A. |
hierarchical clustering |
[cgc6390]:Cluster_A
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Transcripts that showed significantly decreased expression after animals were treated with 50uM Rifampicin and 100uM Psora from day 1 to day 3 adult hermaphradite. |
DESeq2(v1.14.1), fold change > 2, p-value < 0.05 |
WBPaper00055354:Rifampicin-Psora_downregulated
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Genes enriched in intestine. |
To identify genes that are significantly enriched by mRNA tagging, we first normalized the total amount of Cy3 and Cy5 signal to each other in each hybridization. We measured the ratio of the signals from the co-immunoprecipitated mRNA (Cy5) to total RNA in the cell extract (Cy3), and calculated the percentile rank for each gene relative to all genes in each hybridization. The mean percentile rank was determined from eight repeats of the mRNA-tagging experiment. Student's t-test was used to determine which genes showed a mean enrichment significantly greater than the median enrichment for all genes (P<0.001). |
WBPaper00026980:intestine_enriched
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Genes that showed decreased expression after germline ablation comparing to un-ablated animals. |
The differential expression between germline-ablated versus gonad-ablated animals was computed via the functions makeContrasts and contrasts.fit in the limma package in R/Bioconductor. |
WBPaper00045571:germline-ablation_downregulated
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Bacteria: Pseudomonas donghuensis HYS |
Transcripts that showed significantly increased expression after 24 hours of infection by Pseudomonas donghuensis HYS, comparing to control animals exposed to OP50. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00068216:HYS_vs_OP50_upregulated
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Bacteria: Pseudomonas donghuensis HYS |
Transcripts that showed significantly decreased expression after 24 hours of infection by Pseudomonas donghuensis HYS with deletion of ferric uptake regulator (FUR), comparing to control animals exposed to HYS. |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00068216:FUR_vs_HYS_downregulated
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Transcripts that showed significantly decreased expression in skn-1(lax188);daf-2(e1370) comparing to in skn-1(lax188) animals |
DESeq2, fold change > 2, FDR < 0.05. |
WBPaper00067765:daf-2(e1370)_downregulated_skn-1(lax188)
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Genes up or down regulated by 10e-09M of cholesterol . The normalized values used were G/R ratio > 2.6 for up-regulation and G/R ratio < 0.38 for down-regulation, which corresponds to 1.39 and -1.39 log(base2) G/R ratio, respectively. |
The normalized values used were: G/R ratio > 2.6 for up-regulation and G/R ratio < 0.38 for down-regulation, which corresponds to 1.39 and -1.39 log(base2) G/R ratio, respectively. |
WBPaper00005124:cholesterol_10-9M_regulated
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Class IV genes are down-regulated at 6 h, but not at earlier time points. |
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WBPaper00006465:IV_ethanol_late_repression
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Bacteria infection: Burkholderia pseudomallei BpR15 |
Genes that showed decreased expression in adult animals after 12 hour exposure to B. pseudomallei R15 vs. exposure to OP50 |
After array normalization, significance analysis of microarray was performed on data from individual time points (two class unpaired response) to identify genes with small but consistent changes. A false-discovery rate (FDR) of ~1% was used as criteria to consider a gene differentially regulated.Authors chose this particular FDR because it was the lowest FDR that did not significantly reduce the number of estimated true positives. |
WBPaper00044091:Bpseudomallei_repressed_12h
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Transcripts that showed significantly decreased expression (fold change > 2, adjusted p-value < 0.05) in EKM99[met-2(n4256) set-25(n5021)] injected with vector RNAi, comparing to in control animals (N2 injected with vector RNAi). |
Differential expression analysis was performed using DESeq2 v1.6.3 in R version 3.2.3. All analyses were performed with genes that had average expression level above 1 RPKM (fragments per kilobase per million, as calculated by Cufflinks). |
WBPaper00050193:met-2(n4256)set-25(n5021)_downregulated_L1
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Transcripts that showed significantly decreased expression in hlh-26(ok1453) animals exposed to E. faecium for 8 hours, comparing to N2 animals exposed to E. faecium for 8 hours. |
Fold change > 2. |
WBPaper00062585:hlh-26(ok1453)_downregulated_E.faecium
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Transcripts that showed significantly decreased expression after animals were exposed to 500 uM of 3-dihydro-2-phenyl-sulfanylenebenzo[d] [1,3,2]-oxazaphosphole (FW1256) from L1 stage larvae onwards. |
The DESeq2 package (v1.24.0) was used to identify differentially expressed genes (DEGs). Fold change > 2, FDR < 0.05. |
WBPaper00061040:FW1256_downregulated
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hermaphrodite soma-enriched |
Comparisons were made between genotypes by subtracting the mean log value of one ratio from another, and the significance of the difference was evaluated using Student t-test for two populations. For the fem-3(gf) versus fem-1(lf) direct comparison, authors performed the same analysis, except they used a Students t-test for one population. Author chose a combination of a twofold difference with a t value exceeding 99% confidence (P < 0.01), because these criteria allowed the inclusion of essentially all genes that had previously been identified as germline-enriched in a wt/glp-4 hermaphrodite comparison. Additionally, requiring a twofold difference reduced false positives, as the number of genes with two-fold difference and a P<0.01 only included ~100 genes more than with P < 0.001, and almost all genes showed germline expression by in situ hybridization. |
[cgc6390]:herm_soma-enriched
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Genome-wide analysis of developmental and sex-regulated gene expression profile. |
self-organizing map |
cgc4489_group_4
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Transcripts of noncoding genes that showed significantly decreased expression in muscle. |
DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. |
WBPaper00062325:muscle_depleted_noncoding-RNA
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Bacteria infection: Burkholderia pseudomallei BpR15 |
Genes that showed decreased expression in adult animals after 8 hour exposure to B. pseudomallei R15 vs. exposure to OP50 |
After array normalization, significance analysis of microarray was performed on data from individual time points (two class unpaired response) to identify genes with small but consistent changes. A false-discovery rate (FDR) of ~1% was used as criteria to consider a gene differentially regulated.Authors chose this particular FDR because it was the lowest FDR that did not significantly reduce the number of estimated true positives. |
WBPaper00044091:Bpseudomallei_repressed_8h
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