2 Contained In
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. | L4 larva Ce | WBls:0000038 | ||
| A C. elegans life stage that occurs during the L4 larval life stage | C. elegans life stage occurring during L4 larval stage | WBls:0000821 |
6 Expression Patterns
| Remark | Reporter Gene | Primary Identifier | Pattern | Subcellular Localization |
|---|---|---|---|---|
| Expr16423 | We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline. | |||
| Expr12305 | Only reporter in this study where expression was obviously turned off during L4. | |||
| Expr12300 | Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7. | |||
| Expr12299 | Variable expression in L4.1; consistent in later sub-stages. | |||
| Expr12306 | Variable expression possibly due to loss of extrachromosomal array. | |||
| Expr16585 | We examined NHR-23::GFP expression over the course of the 4th larval stage, as vulva morphology allows for more precise sub-staging (Mok et al., 2015). NHR-23::GFP expression was first detectable in L4.1 larvae, peaked in expression from L4.2-L4.3, disappeared in the vulva by L4.5 and in the head by L4.6, and then remained undetectable, similar to what the observations of Kinney et al. (2023) of NHR-23::mScarlet. |
1 Followed By
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The third sub-stage of the L4 larva. All vulval cells complete migration. vulA, vulC, and vulD undergo cell fusion. The vulval lumen has widened and a prominent kink has formed between vulC and vulD. The uterine lumen has not started to form. At 20 Centigrade: 36.8 hours after hatching, 2.8 hours from the L3-to-L4 molt. | L4.2 larva Ce | WBls:0000685 |
1 Preceded By
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The first sub-stage of the L4 larva. All vulval cells are migrating toward the center of the future vulva. vulA, vulB, and vulE have divided, but vulC and vulF have not. At 20 Centigrade: 34.3 hours after hatching, 0.3 hours from the L3-to-L4 molt. | L4.0 larva Ce | WBls:0000683 |
