WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Life Stage :

Definition  The third sub-stage of the L4 larva. All vulval cells complete migration. vulA, vulC, and vulD undergo cell fusion. The vulval lumen has widened and a prominent kink has formed between vulC and vulD. The uterine lumen has not started to form. At 20 Centigrade: 36.8 hours after hatching, 2.8 hours from the L3-to-L4 molt. Primary Identifier  WBls:0000685
Public Name  L4.2 larva Ce
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0 Anatomy Terms

2 Contained In

Remark Definition Other Name Public Name Primary Identifier
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  A C. elegans life stage that occurs during the L4 larval life stage C. elegans life stage occurring during L4 larval stage WBls:0000821

0 Expression Clusters

9 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr16423 We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.  
    Expr12298    
    Expr12304    
    Expr12305 Only reporter in this study where expression was obviously turned off during L4.  
    Expr15947   The transmembrane eLRRon protein LET-4 marked all vulval apical membranes during the late L4.2-L4.7 period, and thereafter appeared intracellular.
    Expr12300 Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7.  
    Expr12299 Variable expression in L4.1; consistent in later sub-stages.  
    Expr12306 Variable expression possibly due to loss of extrachromosomal array.  
    Expr16585 We examined NHR-23::GFP expression over the course of the 4th larval stage, as vulva morphology allows for more precise sub-staging (Mok et al., 2015). NHR-23::GFP expression was first detectable in L4.1 larvae, peaked in expression from L4.2-L4.3, disappeared in the vulva by L4.5 and in the head by L4.6, and then remained undetectable, similar to what the observations of Kinney et al. (2023) of NHR-23::mScarlet.  

1 Followed By

Remark Definition Other Name Public Name Primary Identifier
  The fourth sub-stage of the L4 larva. vulFs have separated and the apex of the lumen is flat and capped by the anchor cell. The uterine lumen starts to form. At 20 Centigrade: 38.1 hours after hatching, 4.1 hours from the L3-to-L4 molt. L4.3 larva Ce WBls:0000686

1 Preceded By

Remark Definition Other Name Public Name Primary Identifier
  The second sub-stage of the L4 larva. The inner cells of the vulva have finished migrating and have formed toroids, but have not fused. vulC and vulF have divided and a narrow lumen has formed. At 20 Centigrade: 35.1 hours after hatching, 1.1 hours from the L3-to-L4 molt. L4.1 larva Ce WBls:0000684

0 Sub Stages