2 Children
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Pharyngeal gland cell 2L. | g2L | lineage name: MSaapapaa | WBbt:0004526 |
| Pharyngeal gland cell 2R. | g2R | lineage name: MSpapapaa | WBbt:0004524 |
4 Expression Patterns
| Remark | Reporter Gene | Primary Identifier | Pattern | Subcellular Localization |
|---|---|---|---|---|
| Expr11217 | kpc-1 is expressed in numerous neuronal and epithelial cells during dauer. Processes adjacent to the body wall in dauers resemble those of IL2Q 4 dendrites. To determine whether kpc-1 is expressed in the IL2s, we coinjected the Pkpc-1::GFP reporter with a Pklp-6::tdTomato reporter that is expressed exclusively in the IL2s. During nondauer stages, kpc-1 is not expressed in the IL2 neurons (data not shown). In dauers, Pkpc-1::GFP and Pklp-6::tdTomato are coexpressed, indicating that kpc-1 is upregulated in the IL2s during dauer. Nondauer expression was consistently observed in the ventral nerve cord and pharynx with strong expression in the g2 pharyngeal gland cells and vpi pharyngeal intestinal valve cells. | |||
| Other strains-- UL856, UL853 | Expr162 | Strong expression is seen in the excretory cells and canals. Expression in the canals is concentrated in unidentified subcellular structures. Expression is also seen in the terminal bulb of the pharynx, possibly in the g2 gland cell. Expression is most frequently seen in young larvae, but is occasionally seen in embryos and adults. | ||
| Expr11043 | F48A11.1 was expressed specifically in the period immediately preceding each moult. Expression appeared limited to the pharynx and specifically to the glandular cells g1 and g2 (5 nuclei) in the terminal bulb, the three m4 myo-epithelial cells (6 nuclei) in the metacorpus and the three m3 myo-epithelial cells (6 nuclei) in the pro- corpus. No GFP was detected elsewhere in transgenic nematodes despite careful examination of the reproductive tissues and intestine. | |||
| Expr16579 | Using a promoter immediately upstream of the start codon of the eight longest pezo-1 isoforms, we drove expression of GFP in several mesodermal tissues, including the body wall and pharyngeal muscles, pharyngeal glands and valve, and uterine and defecation tissues. Our findings are consistent with those described in a recent preprint by Millet et al. (2021). Based on their unique morphology and location, we identified GFP expression in pharyngeal muscles (pm3-8), pharyngeal glands (dg1, vg1s, vg2s), pharyngeal-intestinal valve (vpi) cells, AMsh, and AMso glial cells. We also tentatively identified pharyngeal neurons M1 and I4. Medially, we saw labeling of the HSN neuron, the vulval muscles, uterine muscles, and intestine. Posteriorly, we saw labeling in cells involved in defection. Based on their morphology and position, we tentatively identified these as rectD, K, F, B, U, and Y (N1⁄421). In addition to GFP-based transcriptional reporters, we used the CRISPR/Cas9-generated AG467 (integrated) strain which used mScarlet to tag the C terminus of PEZO-1 proteins. Imaging of AG467 (N 1⁄4 6) showed PEZO-1 proteins localized to the vicinity of the pharyngeal grinder, to the quadrants of the pharyngeal bulb occupied by the gland cells, and to the tip of the nose of the worm where the amphid sheath is located. We conclude that reporters upstream of long pezo-1 isoforms drive gene expression largely in muscles and other mesodermal tissues, although some neuronal expression was also seen. |
12 Life Stages
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. | embryo Ce | WBls:0000003 | ||
| The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. | fully-elongated embryo Ce | WBls:0000021 | ||
| The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. | elongating embryo Ce | WBls:0000015 | ||
| The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. | enclosing embryo Ce | WBls:0000013 | ||
| The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation. | gastrulating embryo Ce | WBls:0000010 | ||
| The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over. | proliferating embryo Ce | WBls:0000004 | ||
| The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo. | comma embryo Ce | WBls:0000017 | ||
| The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. | 2-fold embryo Ce | WBls:0000019 | ||
| The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. | 3-fold embryo Ce | WBls:0000020 | ||
| The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo. | 1.5-fold embryo Ce | WBls:0000018 | ||
| The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage. | bean embryo Ce | WBls:0000016 | ||
| The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes. | late cleavage stage embryo Ce | WBls:0000014 |
2 Parents
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Secretory gland cell of the pharynx | Pharyngeal gland cell | WBbt:0005788 | |
| the last, posterior bulb of the pharynx. | terminal bulb | WBbt:0003732 |
