WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  Using a promoter immediately upstream of the start codon of the eight longest pezo-1 isoforms, we drove expression of GFP in several mesodermal tissues, including the body wall and pharyngeal muscles, pharyngeal glands and valve, and uterine and defecation tissues. Our findings are consistent with those described in a recent preprint by Millet et al. (2021). Based on their unique morphology and location, we identified GFP expression in pharyngeal muscles (pm3-8), pharyngeal glands (dg1, vg1s, vg2s), pharyngeal-intestinal valve (vpi) cells, AMsh, and AMso glial cells. We also tentatively identified pharyngeal neurons M1 and I4. Medially, we saw labeling of the HSN neuron, the vulval muscles, uterine muscles, and intestine. Posteriorly, we saw labeling in cells involved in defection. Based on their morphology and position, we tentatively identified these as rectD, K, F, B, U, and Y (N1⁄421). In addition to GFP-based transcriptional reporters, we used the CRISPR/Cas9-generated AG467 (integrated) strain which used mScarlet to tag the C terminus of PEZO-1 proteins. Imaging of AG467 (N 1⁄4 6) showed PEZO-1 proteins localized to the vicinity of the pharyngeal grinder, to the quadrants of the pharyngeal bulb occupied by the gland cells, and to the tip of the nose of the worm where the amphid sheath is located. We conclude that reporters upstream of long pezo-1 isoforms drive gene expression largely in muscles and other mesodermal tissues, although some neuronal expression was also seen. Primary Identifier  Expr16579
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28 Anatomy Terms

Definition Name Synonym Primary Identifier
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine. pharyngeal-intestinal valve cardia WBbt:0005767
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
type of cells that make up muscle layers in the pharynx. pharyngeal muscle cell   WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic HSNL lineage name: ABplapppappa WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic HSNR lineage name: ABprapppappa WBbt:0004757
Secretory gland cell of the pharynx Pharyngeal gland cell   WBbt:0005788
muscle lining of the uterine wall. uterine muscle   WBbt:0005342
Embryonic cell of pedigree ABplaapaapp, amphid sheath cell, left. AMshL ABplaapaapp WBbt:0003935
Embryonic cell of pedigree ABpraapaapp, amphid sheath cell, right. AMshR ABpraapaapp WBbt:0003933
seventh pharyngeal muscle cell layer pm7 m7 WBbt:0003721
eighth pharyngeal muscle cell layer (single cell) pm8 lineage name: MSaaapapp WBbt:0004302
fifth pharyngeal muscle cell layer pm5 M5 WBbt:0003737
fourth pharyngeal muscle cell layer pm4 M4 WBbt:0003739
third pharyngeal muscle cell layer pm3 M3 WBbt:0003740
Rectal cell, postembryonic blast cell in male B cell lineage name: ABprppppapa WBbt:0003825
Rectal cell, blast cell in male F cell lineage name: ABplppppapp WBbt:0004799
Rectal epithelial cells, adjacent to intestino-rectal valve, have microvilli rect_D lineage name: ABplpappppp WBbt:0004788
sixth pharyngeal muscle cell layer pm6 m6 WBbt:0003724
Rectal cell, postembryonic blast cell in male U cell lineage name: ABplppppapa WBbt:0004942
Rectal cell, postembryonic blast cell K cell lineage name: ABplpapppaa WBbt:0004499
Pharyngeal gland cell 2. G2   WBbt:0003710
Pharyngeal gland cell 1. G1   WBbt:0003712
Neuron class of one pharyngeal motorneuron. M1 neuron lineage name: MSpaapaaa WBbt:0004488
Neuron class of one pharyngeal interneuron. I4 neuron I4 WBbt:0004741
Rectal cell at hatching, becomes PDA in hermaphrodite, postembryonic blast cell in male Y cell lineage name: ABprpppaaaa WBbt:0004578
epithelial support cell of the amphid sensilla. amphid socket cell AMso WBbt:0008379

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00007505 pezo-1 C10C5.1 Caenorhabditis elegans

0 Life Stages