WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00087516 Phenotype Remark  While the uteri of wild-type control hermaphrodites were filled with rapidly dividing embryos of many different ages, the uteri of hermaphrodites that had been injected with double stranded Ce-cdc-27 RNA were filled with one-cell embryos. These cdc-27(RNAi) embryos looked like those from mat-1 mothers when examined either in utero or as individual fixed specimens that had been processed for DAPI staining and tubulin immunofluorescence. The mat-1 and cdc-27(RNAi) embryos accumulated in an arrested but otherwise normal metaphase I state. In all cases, the oocyte chromosomes were aligned in a pentagonal metaphase plate on a barrel-shaped meiotic spindle, and the sperm chromosomes remained highly condensed. Such embryos also lacked polar bodies (the discarded products of the meiotic divisions) and had incompletely hardened eggshells.
Remark  paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003132 mat-1 Y110A7A.17 Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:Y110A7A.17a Y110A7A.17a 2367   I: 5124192-5124346
CDS:Y110A7A.17b Y110A7A.17b 2373   I: 5124192-5124346

1 Laboratories

Primary Identifier
AG

2 Phenotype

Identifier Name Description
WBPhenotype:0001100 early embryonic lethal Animal dies prior to the gastrulation stage of embryogenesis.
WBPhenotype:0000372 no polar body formation Animals lack the two small cells produced, each during one of the two meiotic cell divisions (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005823

1 Strain

WormBase ID
WBStrain00000001