WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00087536 Phenotype Remark  cel-1(RNAi) embryos arrested development after forming ~100 cells that lacked any signs of differentiation. This terminal arrest phenotype is very similar to that observed when the pol II large subunit or various other broadly essential mRNA transcription factors are inhibited by RNAi. However, early cell division timing and cleavage planes were normal in cel-1(RNAi) embryos, suggesting that these embryos contained appropriate maternal mRNA stores. One abnormality was the cell cycle period of the endodermal precursor cells Ea and Ep, which was shortened compared with wild type. In cel-1(RNAi) embryos, total levels of the pol II large subunit AMA-1 are unaffected, but CTD phosphorylation was highly abnormal. As in wild type embryos, in cel-1(RNAi) embryos Ser-5 phosphorylation was detectable as bright punctate staining pattern in somatic nuclei. In contrast, in cel-1(RNAi) embryos levels of specific alpha-Ser(P)-2 staining were dramatically reduced, to a level only slightly higher than the background observed in ama-1(RNAi) embryos. Levels of the CTD Ser-2 kinase CDK-9 appeared to be normal in cel-1(RNAi) embryonic nuclei, arguing that the drop in CTD phosphorylation was not an indirect effect. The specific and substantial defect in CTD Ser-2 phosphorylation suggests that when CEL-1 levels are depleted, the normal progression of CTD phosphorylation during transcription is disrupted at most or possibly all genes.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000466 cel-1 C03D6.3 Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:C03D6.3a C03D6.3a 1872   I: 9665311-9665391
CDS:C03D6.3b C03D6.3b 1722   I: 9665347-9665391

0 Laboratories

2 Phenotype

Identifier Name Description
WBPhenotype:0000867 embryonic arrest Cessation of development during any stage occurring after fertilization and until hatching compared to control.
WBPhenotype:0001115 cell cycle timing defective early emb Cell cycle timing is abnormal during the first four cell divisions.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005845

0 Strain