WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00101329 Genotype  MIG-14::GFP; LMP-1::mCherry
Phenotype Remark  In control animals there is no overlap between MIG-14::GFP and LMP-1::mCherry expression in the cell. In vps-35(RNAi) animals, however, most of the MIG-14::GFP protein that remains is localized to LMP-1::mCherry-positive structures. The LMP-1::mCherry fusion protein is expressed in lysosomes. Subcellular localization of MIG-14::GFP in control animals was throughout the cell and at the plasma membrane. Remark  Exact sequence used for RNAi not stated by authors, Vidal laboratory clone used for curation. Authors state RNAi in was performed by feeding using clones from the Ahringer or Vidal libraries.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

2 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00006933 vps-35 F59G1.3 Caenorhabditis elegans
WBGene00019129 egc-3 F59G1.8 Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:F59G1.8a F59G1.8a 606   II: 5906592-5906678
CDS:F59G1.8b F59G1.8b 615   II: 5906592-5906678
CDS:F59G1.3 F59G1.3 2466   II: 5907820-5907924

1 Laboratories

Primary Identifier
KN

1 Phenotype

Identifier Name Description
WBPhenotype:0000679 transgene subcellular localization variant Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00045530

0 Strain