WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00101326 Genotype  mig-1(e1787) lin-17(n677); cfz-2(ok1201); mec-4::mom-5(RNAi); PLR-1-mCherry
Phenotype Remark  PLR-1 accumulates at the surface in this background. Normally PLR-1 is enriched in endosomes and not detected at the cell surface, that is in wild-type, mec-4::mom-5(RNAi) and mig-1 lin-17; cfz-2 triple mutant animals, PLR-1 exhibits a punctate localization pattern (not detected at the surface). It is only in mig-1(e1787) lin-17(n677); cfz-2(ok1201); mec-4::mom-5(RNAi) that surface accumulation is observed. Thus, elimination or reduction of endogenously expressed Frizzleds causes cell-surface accumulation of PLR-1. Remark  mec-4 promoter was used for specific RNAi of mom-5 in mec-4 expressing cells to circumvent lethality caused by mom-5 RNAi in the entire animal. Exact sequence used for RNAi not stated by authors, exon 1 used for curation.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003397 mom-5 T23D8.1 Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:T23D8.1a T23D8.1a 1707   I: 9965641-9966113
CDS:T23D8.1b T23D8.1b 1713   I: 9965641-9966113

1 Laboratories

Primary Identifier
SK

1 Phenotype

Identifier Name Description
WBPhenotype:0000679 transgene subcellular localization variant Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00044679

0 Strain