WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00106299 Phenotype Remark  "... we first compiled a list of C-elegans chaperones (97 genes) (Table 1) and examined the effects of down-regulation of each chaperone in wild-type animals (Figure 2A). Down-regulation of genes encoding 10 chaperones (17 genes), including CTT, HSP-1 (Hsc70/HSPA8), HSP-4 (BIP), HSP-6 (Mortalin), and DAF-21 (Hsp90), resulted in severe organismal phenotypes, such as lethality, developmental arrest, or sterility (Table 1, Figure S2). In contrast, downregulation of genes encoding most chaperones examined had no apparent phenotypes (Table 1). Our data are in agreement with published RNAi screens for wild-type RNAi treatment (Table 1, data curated by wormbase). When knockout mutants or data from enhanced RNAi efficacy experiments, (for example via dsDNA [sic] injection into the gonads) were considered, more acute phenotypes were observed for some chaperones (Table 1, data curated by wormbase)." "As expected, down-regulation of only two chaperones, unc45 or unc-23, showed a marked movement defect. For example, based on their impact on muscular ultrastructure or their involvement in myopathies, molecular chaperones, such as TRIC (cct), alpha-B-crystallin/HSPB5 (hsp-25), and DNAJB6 (dnj-24), were suggested to play a key role in sarcomere assembly and maintenance (Ding and Candido, 2000; Vang et al., 2005; Kim et al., 2008; Willis et al., 2009; Willis and Patterson, 2010; Garrido et al., 2012; Haslbeck et al., 2012; Sarparanta et al., 2012). Although these chaperones are associated with the sarcomere (Leroux and Candido, 1997; Ding and Candido, 2000; Meissner et al., 2011), knockdown of their genes by feeding RNAi did not result in notable motility defects (Table 1)."
Remark  (Table 1) cct-2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000378 cct-2 T21B10.7 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:T21B10.7 T21B10.7 1590   II: 8927745-8927873

0 Laboratories

2 Phenotype

Identifier Name Description
WBPhenotype:0000688 sterile Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce.
WBPhenotype:0000059 larval arrest The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00046852

1 Strain

WormBase ID
WBStrain00000001