WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00106396 Genotype  unc-45(e286)
Phenotype Remark  "RNAi knockdown of chaperones was next performed with unc-45(ts) mutant strains under permissive conditions. Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15°C) were treated with RNAi for different molecular chaperones and monitored for motility defects. We first sought chaperones that cause unc-45(e286)-specific phenotypes at permissive conditions when depleted (Figure 2A). Of the 96 RNAi constructs tested, five genes induced loss of coordination and/or an egg-laying defect, resulting in reduced motility in more than 70% of the unc-45(e286) mutant animals. As noted above, down-regulation of unc-45 or unc-23 also induced a loss of coordination in wild-type animals (Table 1); these were discarded from the candidate gene list (Figure 2A). The remaining chaperones identified in the screen are CeHsp90 (daf-21), CeHop (sti-1) and CeAha1 (C01G10.8) (Figures 2B,C)." "CeHop and CeAha1 RNAi only affected unc45(e286)-expressing animals, where complete paralysis resulted (Figures 2B,C)." Remark  (Figure 2) sti-1/CeHop RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00019983 sti-1 R09E12.3 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:R09E12.3 R09E12.3 963   V: 773591-773602

0 Laboratories

1 Phenotype

Identifier Name Description
WBPhenotype:0000644 paralyzed Animals do not move and are not responsive to external mechanical stimulation.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00046852

0 Strain