WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00088417 Phenotype Remark  Whereas ~8% of the F1 progeny arrested as unhatched embryos, the majority of progeny arrested as larvae in this case (37% L1-L2 arrest, and 22% L3-L4 arrest, n=328). The variable phenotypes observed were probably due to gene-dependent inefficacy inherent of RNAi experiments. These larvae showed no apparent morphological abnormality, but were affected severely in locomotion. Even F1 progeny that arrested as unhatched embryos seemed to have completed normal morphogenesis. Because expression of COH-1 became detectable at the mid-embryogenesis stage, and the RNAi-arrested embryos showed a significantly reduced level of COH-1, it seemed that the most rigorous loss-of-function phenotype for coh-1 would be late embryonic lethality after completion of morphogenesis. Authors next disrupted the function of COH-1 postembryonically by soaking L1 worms to a dsRNA solution and found that some coh-1(RNAi) adults were defective in egg laying. The observed defects in locomotion and egg laying implicated that the function of COH-1 might be important for proper function of muscle and/or the nervous system.
Remark  paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. Authors used yk226d1 (coh-1).
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000591 coh-1 K08A8.3 Caenorhabditis elegans

4 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:K08A8.3c K08A8.3c 414   X: 7466321-7466491
CDS:K08A8.3b K08A8.3b 495   X: 7466321-7466491
CDS:K08A8.3a K08A8.3a 1794   X: 7466321-7466491
CDS:K08A8.3d K08A8.3d 156   X: 7466321-7466476

1 Laboratories

Primary Identifier
YM

4 Phenotype

Identifier Name Description
WBPhenotype:0000867 embryonic arrest Cessation of development during any stage occurring after fertilization and until hatching compared to control.
WBPhenotype:0000643 locomotion variant Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals.
WBPhenotype:0000006 egg laying defective Eggs are not laid, laid at a slower rate or eggs are laid at a later stage of development compared to control.
WBPhenotype:0000059 larval arrest The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005937

1 Strain

WormBase ID
WBStrain00000001