WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00088516 Genotype  clr-1(e1745)
Phenotype Remark  In a clr-1(e1745) genetic background that allows the visualization of cell boundaries, cul-4 RNAi produced a marked increase in the size of seam cells. However, the size of nuclei in the non-proliferative hyp7 syncytial cell8 was unaffected by inactivation of cul-4. Larger cells were also observed among other cul-4 RNAi blast cell lineages, including the M, Q, P and somatic gonad cell lineages. Germ cells did not become enlarged. However, germ cells often entered meiosis prematurely to become sperm in the L2 larval stage; this is in contrast to the wild type, in which spermatogenesis occurs in the L4 larval stage. Authors measured the amount of genomic DNA in enlarged cul-4 RNAi blast cells and observed markedly elevated DNA levels. Two to three days post-hatch, cul-4 RNAi seam cells contain up to 100C DNA content, compared with the 2C of the wild type. The increased ploidy seems to be caused by DNA re-replication. Remark  paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. yk34c8 was used by authors.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000839 cul-4 F45E12.3 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:F45E12.3 F45E12.3 2523   II: 7302111-7302212

1 Laboratories

Primary Identifier
ET

2 Phenotype

Identifier Name Description
WBPhenotype:0000533 cell morphology variant Any variation in the form or composition of the basic structural and functional unit of all organisms compared to control. Includes the plasma membrane and any external encapsulating structures such as the cell wall and cell envelope.
WBPhenotype:0000433 DNA synthesis variant Variations in the processes involving the production of new DNA strands compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00005912

0 Strain