WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00107817 Phenotype Remark  Figure 2, mtm-1 RNAi shows phagosome maturation defect. "... mtm-1(lf) phagosomes were not stained by LysoSensor green, indicating defects in phagosomal acidification (Fig 2, C-D' and O). Moreover, phagosomal association of LST-4, DYN-1, RAB-5, and RAB-7, which are recruited at early or late maturation stages, were all disrupted, and the lysosome reporter NUC-1 was also absent from phagosomes in mtm-1 RNAi animals (Fig 2, E-O; Guo et_al, 2010). In contrast, phagosomal labeling by PLCδ1-PH was significantly increased in mtm-1(lf) worms (Fig 2, A-B' and O). These data indicate that apoptotic cell-containing phagosomes are arrested very early at the PtdIns(4,5)P2-positive stage in mtm-1(lf) worms... In mtm-1(qx322) or mtm-1 RNAi worms, almost all germ cell corpses were labeled by YFP::2xFYVE, whereas <50% of them were YFP positive in wild type (Fig 5, A-C' and G). Moreover, extending pseudopods, which are positive for PLCδ1-PH and MTM-1(C378S) but not 2xFYVE in wild type, were labeled by YFP::2xFYVE in mtm-1(lf) (Fig S3 J)... Surprisingly, loss of mtm-1, which led to increased PtdIns3P accumulation on plasma membranes and forming phagosomes, also abrogated LST-4 recruitment (Fig 2, K-O; Fig 6 D; and Fig S4 H)."
Remark  mtm-1 RNAi
Paste the following link
Lists
This RNAi isn't in any lists. Upload a list.

1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003475 mtm-1 Y110A7A.5 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:Y110A7A.5 Y110A7A.5 1767   I: 5134767-5134806

0 Laboratories

1 Phenotype

Identifier Name Description
WBPhenotype:0001846 phagosome maturation defective Early phagosomes fail to undergo the maturation process following the ingestion of particulate material by phagocytosis; During maturation, phagosomes acquire markers of late endosomes and lysosomes.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00048406

0 Strain