WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00107819 Phenotype Remark  Figure 2, ocrl-1 RNAi shows phagosome maturation defect; "Inactivation of OCRL-1, the inositol 5-phosphatase that hydrolyzes PtdIns(4,5)P2, caused prolonged MTM-1(C378S) duration on phagosomes like PPK-1 expression (see Fig 4, F and G)... Consistent with the sealing defect, ocrl-1(lf) phagosomes were not acidified, and the recruitment of maturation factors (RAB-5 and RAB-7) and lysosomes (NUC-1) was blocked (Fig 2, R-U' and X)... RNAi inactivation of the inositol 5-phosphatase OCRL-1 led to sustained MTM-1(C378S) duration on phagosomes (Fig 4 G)... the appearance of 2xFYVE on phagosomes was severely impaired, suggesting that sustained MTM-1 suppresses PtdIns3P accumulation (Fig 4, B, D, and G)... Overexpression of PPK-1 or inactivation of OCRL-1, which causes increased PtdIns(4,5)P2 on phagosomes, led to extended LST-4 duration accompanied by defective 2xFYVE appearance (Fig 2, V-X; Fig 6 C; and Fig S4, B-F)."
Remark  ocrl-1 RNAi
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00007620 ocrl-1 C16C2.3 Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:C16C2.3a C16C2.3a 2139   I: 9715633-9715757
CDS:C16C2.3b C16C2.3b 816   I: 9715633-9715757

0 Laboratories

1 Phenotype

Identifier Name Description
WBPhenotype:0001846 phagosome maturation defective Early phagosomes fail to undergo the maturation process following the ingestion of particulate material by phagocytosis; During maturation, phagosomes acquire markers of late endosomes and lysosomes.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00048406

0 Strain