WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00107831 Genotype  GFP::LGG-1
Phenotype Remark  "Since many tumors require drastic changes in metabolism and particularly in catabolism to grow, we asked whether autophagy is induced in germline tumors in C. elegans. To test this, we examined whether autophagy-related proteins are expressed in the wild-type gonad and/or in the gld-1 germline tumor. We used transgenic animals that express fluorescence tagged versions of the following proteins: GFP::LGG-1 (GABARAP ortholog), GFP::LGG-2 (LC3 ortholog), and BEC-1::GFP (BECN1 ortholog). Consistent with previous reports, we observed that autophagy-related proteins are highly expressed in the nervous system, pharynx, intestine, hypodermis, vulva, spermatheca and somatic gonad of wild-type animals, but not in wild-type germline cells (Fig 1B). However, cells within the germline tumor in gld-1 RNAi animals show high expression of the autophagy pathway proteins from the first day of adulthood throughout their life span (Fig 1C and D). Notably, these transgenes (with the exception of BEC-1::GFP) are not expressed in wild-type germlines and only become activated during zygotic gene activation in embryos (Fig 1B) or somatic trans-differentiation in the tumor... Importantly, germline tumor cells show a heterogenous pattern of GFP::LGG-1 expression, with many cells showing elevated cytoplasmic expression and about 90% of them contain clearly discernible autophagosomes with rather uniform size of around 0.5 μm (Fig 2B)... Moreover, many (tumorous germ line) cells expressing GFP::LGG-1 have elongated protrusions which are often connected to neighboring cells, thereby resembling neurons in their shape (Fig 2C)." Remark  (Figure 1B-D,2B) gld-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001595 gld-1 T23G11.3 Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:T23G11.3 T23G11.3 1392   I: 7695696-7695884

0 Laboratories

2 Phenotype

Identifier Name Description
WBPhenotype:0001276 ectopic expression transgene Any variation in the cellular expression of a gene product produced by exogenous DNA that has been introduced into the organism such that it now expresses in cells/tissues not seen when compared to the expression of that gene product in control animals.
WBPhenotype:0000533 cell morphology variant Any variation in the form or composition of the basic structural and functional unit of all organisms compared to control. Includes the plasma membrane and any external encapsulating structures such as the cell wall and cell envelope.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00049105

0 Strain