1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
| Pattern | Using the ciliated neuron-specific labeling, we observed robust distribution of endogenous TBB-2 within amphid and phasmid ciliary MTs. Previous attempts using N- terminal GFP::TBB-2 KI allele had masked this phenomenon, likely due to background signals emanating from extraneous tissues. Furthermore, we found that ciliary TBB-2 was predominantly localized within the middle segments of cilia, with diminished signals in the distal segments, thereby revealing a unique and regulated distribution pattern distinct from that of TBB-4 distributing along the full-length cilia. These new findings were previously inaccessible with traditional labeling strategies. The hypodermis-specific labeling facilitated visualizing dynamic architecture of MT network in the hypodermal cell layers, effectively eliminating signal interference from adjacent tissues. The germline-specific labeling enabled tracking of the temporal behavior of mitotic and meiotic MTs within germline as well as in early-stage embryos, excluding background noise emanating from other unrelated tissues. | Primary Identifier | Expr16566 |
| Remark | We genetically crossed the ubiquitously expressed tbb-2 (gfp11-i) KI strain with 3 distinct endogenous gfp1-10 donor elements. These included ciliated neuron-specific che-3::T2A:: gfp1-10 (Pcilium::GFP1-10), hypodermis-specific Pcol-19::gfp1-10 single-copy KI (Phypodermis::GFP1-10), and germline-specific glh-1::T2A::gfp1-10 (Pgermline::GFP1-10). Consequently, TBB-2 was specifically labeled in the ciliated neurons, the hypodermal cell layer, and the germline along with embryos. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006537 | tbb-2 | C36E8.5 | Caenorhabditis elegans |
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