1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006827 | unc-98 | F08C6.7 | Caenorhabditis elegans |
| Primary Identifier | Expr2578 | Remark | EU131 staining was undetectable in the muscle of each of the unc-98 mutants, consistent with the absence of the 37-kDa polypeptide on Western blots, and thus demonstrating the specificity of the antibodies for UNC-98 protein. |
| Subcellular Localization | In wild-type muscle fixed with methanol and paraformaldehyde on whole worms, or alternatively, ethanol or methanol on worm frozen sections, anti-UNC-98 staining was only found at the M-line; no staining was observed at the dense bodies or in nuclei. However, when frozen sections were fixed with n-heptane, staining of M-lines and, in addition, the muscle cell nuclei was observed. Thus, it would seem that there is indeed UNC-98 in the nucleus, but the epitopes detected by these antibodies are inactivated by the usual fixatives. UNC-98 antibodies were used to stain animals genotypically unc-98(su130) that were rescued with a transgenic array carrying the 8.1-kb unc-98rescuing fragment and rol-6 marker DNA. Because extrachromosomal arrays contain multiple expressed copies of an introduced gene, these unc-98-rescued animals are likely to express higher than wildtype levels of UNC-98. In these unc-98-rescued animals, anti-UNC-98 antibodies localize to all three structures labeled with UNC-98-GFP: M-lines, nuclei, and dense bodies. When used against wild-type embryos, these antibodies stain filamentous structures, presumably myofibrils, as early as the 1.5- to 2-fold stage. In wild-type adult animals fixed with methanol and paraformaldehyde, these antibodies stain the M-line region, colocalizing with UNC-89, a previously described component of the M-line region. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00006827 | unc-98 | F08C6.7 | Caenorhabditis elegans |
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