| Pattern | Expression of nhr-49 is detected in the nucleus and cytoplasm of Day 1 adult C. elegans. Fluorescent microscopy of endogenous sfGFP::NHR-49 (long isoform "c", nhr-49c(syb9651[sfGFP::NHR-49c])) shows nuclear enrichment with additional cytoplasmic localization above autofluorescence in intestinal cells. TurboID proximity labeling indicates isoform- and tag-specific localization patterns: the N-terminally tagged version (nhr-49(syb10204[NHR-49::3xHA::TurboID]) preferentially labels cytoplasmic and mitochondrial proteins, while the C-terminally tagged version (nhr-49c(syb10203[3xHA::TurboID::NHR-49c]) enriches for nuclear and nuclear pore components. Western blot indicates low steady-state levels of the long isoform at Day 1. These findings suggest distinct isoform-specific expression dynamics for nhr-49. | Primary Identifier | Expr16605 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003639 | nhr-49 | K10C3.6 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| At 20 Centigrade: 24-48 hours after L4-adult molt. 4-5 days after first cleavage. | 1-day post-L4 adult hermaphrodite Ce | WBls:0000064 |
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