1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003582 | dcap-2 | F52G2.1 | Caenorhabditis elegans |
| Primary Identifier | Expr3746 | Subcellular Localization | Immunostaining of C. elegans embryos revealed that Dcp2 protein was found enriched in particles in the embryonic cytoplasm, and these particles disappear in embryos depleted of Dcp2 using RNAi. Dcp2 staining particles were on average 0.5 +/- 0.03 um in size, and many foci were observed per cell. Although it is difficult to count the exact number of foci in a given cell, the number of Dcp2 enriched bodies per cell more closely resembles the many particles observed in mammalian cells than the 2.4 +/- 1.4 foci per cell observed in yeast. Though present around nuclei in the germ cells of the gonad, cytoplasmic (nonperinuclear) Dcp2 foci were first obvious after fertilization and were present by the time the two pronuclei meet and fuse. By the 2-cell stage, Dcp2 bodies were observed throughout the cytoplasm of both the somatic and germ line lineage, though they often appear stronger in the P-cell than the somatic cell at this early point in development. From the 4-cell stage onward, many Dcp2 foci were observed in the cytoplasm of all cells. Dcp2 staining was faint in nuclei. Somatic CGH-1 foci are coincident with Dcp2. In contrast to previous observations showing that CGH-1 somatic foci disappear after the first few embryonic divisions, cytoplasmic Dcp2 foci persist throughout embryonic development. Dcp2 immunostained C. elegans embryos were colabeled with monoclonal antibodies that stain P-granules (K76 and OIC1D4) and also with anti-GLH-1, which has previously been shown to label P-granules. Such immunostaining indicates that Dcp2 is enriched in P-granules. Dcp2 is also present at sites where P-granules are observed in C. elegans germ cells during oogenesis. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003582 | dcap-2 | F52G2.1 | Caenorhabditis elegans |
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