1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003209 | mel-26 | ZK858.4 | Caenorhabditis elegans |
| Primary Identifier | Expr3764 | Remark | Gene_regulation: Strikingly, MEL-26 greatly accumulated at cell-cell contact sites and spread to the cortex in embryos depleted for either CUL-3 or its activator, RFL-1. An accumulation of MEL-26 was also observed by Western blotting of an embryonic extract made from rfl-1(or198) worms shifted to the restrictive temperature. These results confirm that degradation of MEL-26 requires an intact CUL-3 ligase complex and suggest that binding to CUL-3 is not required for its cortical localization. |
| Subcellular Localization | MEL-26 localized to the cytoplasm and accumulated at the site of cell separation during cytokinesis. No staining of cell-cell contact sites was detectable in mel-26(RNAi) embryos, demonstrating that the antibodies are specific for MEL-26. Moreover, a similar expression pattern was observed in embryos expressing a MEL-26::GFP fusion protein. The staining of the centrosomes was nonspecific and varied from one purified antiserum to the other. MEL-26 was not just localized to the membrane region that separates two cells but was also observed on ingressing cleavage furrows in wild-type embryos and accumulated at ectopic ruffles and at sites of upcoming cytokinesis when the CUL-3 complex was impaired. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003209 | mel-26 | ZK858.4 | Caenorhabditis elegans |
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