| Pattern | In wildtype male germlines, RMD-3/6 was first detected in mid-pachytene spermatocytes. Within primary and secondary spermatocytes, RMD-3/6 antibodies labelled discrete structures throughout the cytoplasm that looked similar to that of MSP-containing fibrous bodies. RMD-3/6 subsequently segregated to spermatids in post-anaphase II budding figures (BF), dispersed through the cytosol in spermatids, and localized to the pseudopods of spermatozoa. Because the observed localization patterns suggested that RMD-3/6 was co-localizing with MSP throughout spermatogenesis, we co-labelled male gonads with anti-MSP and anti-RMD-3/6 antibodies. Tight colocalization of RMD-3/6 and MSP was observed at all stages of spermatogenesis. Notably, colocalization was observed in both longitudinal and cross-sectional views of FBs. As previously observed for other sperm proteins, the antibody labeling was brighter in newly budded spermatids than mature spermatids. In newly budded spermatids, MSP and RMD-3/6 showed tight co-localization to the still intact FBs. In mature spermatids, MSP and RMD-3/6 dispersed through the cytoplasm, but their patterns showed only partial co-localization. In spermatozoa, RMD-3/6 co-localized with MSP in the pseudopods. | Primary Identifier | Expr16607 |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Male haploid germ cell. | sperm | spermatozoa | WBbt:0006798 |
| A cell which gives rise to spermatids by meiosis. | spermatocyte | WBbt:0006799 | |
| An immature sperm cell which undergoes morphogenesis to form mature, amoeboid sperm. | spermatid | WBbt:0006800 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00020070 | rmd-6 | R13H9.1 | Caenorhabditis elegans |
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