| Pattern | We used single molecule fluorescence in-situ hybridization (smFISH) to confirm whether endogenous egl-1 mRNA transcripts could be found in URX (JOHNSEN AND HORVITZ 2016). To test the co-localization of the egl-1 fluorescent reporter and endogenous egl-1 mRNA, we used animals that carry the egl-1 reporter transgene nIs343, which expresses nuclear-localized GFP using 6.5 kb of the upstream promoter region of egl-1 (HIROSE AND HORVITZ 2013). These animals showed the same expression pattern as our egl-1 reporter described in Expr14875 (vxIs601), including expression of GFP in a small number of neurons persisting past development, and especially strongly in URX. We found that the GFP expression always (8/8 worms) co-localized with the smFISH signal from probes targeted to the egl-1 transcript. Notably, this expression continued past the L2 larval stage, which is when the last somatic cell deaths in the hermaphrodite occur (SULSTON AND HORVITZ 1977). | Primary Identifier | Expr14876 |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Ring interneuron | URXL | lineage name: ABplaaaaappp | WBbt:0004928 |
| Ring interneuron | URXR | lineage name: ABarpapaappp | WBbt:0004926 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001170 | egl-1 | F23B12.9 | Caenorhabditis elegans |
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