WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  NOS-1 protein is expressed sequentially during embryogenesis. No NOS-1 protein was detected in pregastrulation embryos, even though nos-1 RNA is present in these stages. NOS-1 first appeared in Z2 and Z3 at the 550-cell stage and remained expressed in these cells until the end of embryogenesis. Primary Identifier  Expr1160
Remark  The specificity of antibody was confirmed by staining embryos deficient for NOS-1. Affinity-purified anti-NOS-1 antibody detected NOS-1 in nos-2(RNAi) embryos but not in nos-1(RNAi) and nos-1(gv5) embryos. NOS-1 and NOS-2 thus do not depend on each other for expression. To test whether NOS-1 is expressed zygotically, authors crossed nos-1(gv5) hermaphrodites (which express no NOS-1) to wild-type males and stained their progeny for NOS-1 protein. Detected NOS-1 expression in a pattern identical to that observed in embryos derived from wild-type hermaphrodites, confirming that NOS-1 expression is zygotic.
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2 Anatomy Terms

Definition Name Synonym Primary Identifier
Germ line precursor cell Z3 lineage name: P4.a WBbt:0004575
Germ line precursor cell Z2 lineage name: P4.p WBbt:0004576

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003783 nos-1 R03D7.7 Caenorhabditis elegans

4 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015
  The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. enclosing embryo Ce WBls:0000013
  The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation. gastrulating embryo Ce WBls:0000010