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Expr16012
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The strongest GFP signals that we identified came from the pharyngeal gland cells. Additionally, we visualized pezo- 1::GFP in a series of cells surrounding the muscle of the corpus and the isthmus (Albertson and Thomson, 1976). We also recognized the arcade cells as putative pezo-1-expressing cells, according to their morphology and location. We also observed many other anterior cells labeled with GFP; however, we cannot currently confirm if they represent neurons and/or amphids.By crossing pezo-1::GFP with a tph-1::DsRed marker-carrying strain, we were able to identify pezo-1 expression in the pha- ryngeal neurosecretory, motor, and sensory (propioceptive/ mechanosensory) neurons (NSML/R). In addition to the pharyngeal cells, we observed expression of pezo-1 in the ventral nerve cord neurons, according to their morphology and location, striated muscles, coelomocytes, spermatheca, vulval muscles, and various male neurons, including the ray neurons. |
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All transgenic lines were screened for dye filling of the amphid and phasmid neurons to confirm rescue of the osm-5(p813) cilia defects and show that the OSM-5GFP fusion protein was functional. |
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Expr857
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Analysis of OSM-5GFP expression reveals almost exclusive localization to the transition zones and cilia of amphids, phasmids, labials and rays of the male tail. |
Expressed in the transitional zone and cilia of sensory neurons. |
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Reporter gene fusion type not specified. |
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Expr864
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Expressed in bean-stage embryo, two-fold elongated embryo, four-fold elongated embryo, L1-L3. Expressed in the nerve ring: more than 8 symmetric pairs of neurons. Expressed in sensory neurons: amphid sensory neurons, labial neurons. Expressed in other neurons: ventral nerve cord, tail neurons. Expressed in non-neuronal tissues: pharynx, vulva. |
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