WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  The protein was absent from somatic cells. Expressed in the gonad. Primary Identifier  Expr2451
Remark  No immunofluorescence was detected after rec-8 RNAi, i.e., in the gonads of F1 worms from embryos laid by animals 24 h or later after dsRNA injection. Also the staining with preimmune serum was negative. Subcellular Localization  In the gonad, REC-8 was localized as small grains all over the nuclei of the mitotic zone, whereas in the transition zone and in pachytene nuclei, it assembled into short and long threads, respectively. In diakinesis, REC-8 was localized along the axes of chromosomes. From metaphase I through to metaphase II REC-8 signals appeared to become progressively less intense or smaller. In metaphase I, both the poleward and equatorial axes of bivalents were decorated with REC-8 whereas in early anaphase I chromosomes, the length of REC-8 immunosignals seemed to be restricted to the original poleward portion of metaphase I chromosomes. In late anaphase I, individual chromosomes could no longer be discriminated and REC-8 spots were visible within the highly condensed DAPI-positive masses of the two half-nuclei. In metaphase II short tracts of REC-8 were observed in the equatorial plane.

1 Anatomy Terms

Definition Name Synonym Primary Identifier
organ producing either sperm or ova. gonad   WBbt:0005175

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00004333 rec-8 W02A2.6 Caenorhabditis elegans

0 Life Stages