WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Primary Identifier  Expr3417 Subcellular Localization  In mated females, extracellular MSP exhibits a graded distribution, with a sharp boundary between the -1 and -2 oocytes. Fluorescence intensity measurements indicate that MSP is localized in a graded manner from the spermatheca to the oocyte. Fluorescence intensity measurements also indicate that there is significant MSP staining over the -2 and -3 oocytes. To pinpoint the localization of MSP at the oocyte cell surface, a 3D confocal analysis of MSP localization was conducted a in mated females using the RME-2 yolk receptor to mark the oocyte plasma membrane and the early endosomal compartments. Three-dimensional image reconstructions of the data indicate that MSP localizes in three regions: (1) in superficial focal planes at the oocyte cell surface with RME-2 just beneath; (2) in the same plane as the RME-2 signal; and (3) within the oocyte beneath the plasma membrane. Using anti-MSP mAbTR-20 and visual inspection, 91% of mated female gonad arms exhibited extracellular MSP localization (n=36), with 39% showing extracellular MSP as far as the most proximal oocyte, and the rest exhibiting extracellular MSP only within the spermatheca. With confocal microscopy, extracellular MSP appeared both punctate and diffuse in the spermatheca, the gonad arm and the uterus. Analysis of 3D data stacks indicated that punctate extracellular MSP was enriched near spermatozoa on the spermathecal walls. The largest puncta were at the diffraction limit of the microscope (0.5 um) and were found nearby spermatozoa. In the proximal gonad arm, MSP was more diffuse and localized in focal plane slices near the oocyte surface. In the uterus, large MSP puncta was observed close to spermatozoa. Diffuse MSP was also observed in extracellular spaces surrounding embryos in the uterus. MSP puncta can be observed near spermatozoa in the uterus and spermatheca using wide-field microscopy, when these regions were less crowded with spermatozoa.
Paste the following link
Lists
This ExpressionPattern isn't in any lists. Upload a list.

6 Anatomy Terms

Definition Name Synonym Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
a female haploid germ cell. oocyte ovum WBbt:0006797
Male haploid germ cell. sperm spermatozoa WBbt:0006798
organ in hermaphrodite animal that produces both ova and sperm. hermaphrodite gonad ovotestis WBbt:0005178
gonad of a male animal, produces sperm. male gonad   WBbt:0006794

47 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003424 msp-3 F26G1.7 Caenorhabditis elegans
WBGene00003430 msp-32 R05F9.3 Caenorhabditis elegans
WBGene00003431 msp-33 R05F9.8 Caenorhabditis elegans
WBGene00003434 msp-38 K08F4.8 Caenorhabditis elegans
WBGene00003442 msp-49 C34F11.6 Caenorhabditis elegans
WBGene00003450 msp-57 R13H9.2 Caenorhabditis elegans
WBGene00003451 msp-58 ZK354.10 Caenorhabditis elegans
WBGene00003456 msp-63 K05F1.7 Caenorhabditis elegans
WBGene00003458 msp-65 ZK354.1 Caenorhabditis elegans
WBGene00003463 msp-76 ZK1251.6 Caenorhabditis elegans
WBGene00003465 msp-78 T13F2.11 Caenorhabditis elegans
WBGene00003466 msp-79 T13F2.10 Caenorhabditis elegans
WBGene00003470 msp-152 ZK546.6 Caenorhabditis elegans
WBGene00003437 msp-42 F26G1.8 Caenorhabditis elegans
WBGene00003435 msp-40 C33F10.9 Caenorhabditis elegans
WBGene00003429 msp-31 R05F9.13 Caenorhabditis elegans
WBGene00003460 msp-71 F59A6.8 Caenorhabditis elegans
WBGene00003462 msp-74 F09C12.7 Caenorhabditis elegans
WBGene00003438 msp-45 F58A6.8 Caenorhabditis elegans
WBGene00003443 msp-50 C34F11.4 Caenorhabditis elegans
WBGene00003469 msp-142 K05F1.2 Caenorhabditis elegans
WBGene00003457 msp-64 ZK1248.6 Caenorhabditis elegans
WBGene00003448 msp-55 C09B9.6 Caenorhabditis elegans
WBGene00003446 msp-53 R13H9.4 Caenorhabditis elegans
WBGene00003426 msp-19 F36H12.7 Caenorhabditis elegans
WBGene00003445 msp-52 F36H12.6 Caenorhabditis elegans
WBGene00003444 msp-51 ZK354.5 Caenorhabditis elegans
WBGene00003468 msp-113 ZK354.4 Caenorhabditis elegans
WBGene00003452 msp-59 ZK354.11 Caenorhabditis elegans
WBGene00003467 msp-81 K07F5.1 Caenorhabditis elegans

0 Life Stages