WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  Immunostaining revealed that the SPN-4 protein was already present in oocytes. The protein was abundant in one-cell-stage embryos. At the two-cell stage, it was present with equal abundance in both blastomeres AB and P1. At the four-cell stage, the protein distribution became different among the blastomeres; strongly in the posterior blastomeres, EMS and P2, and weakly in the anterior blastomeres, ABa and ABp. After the four-cell stage, SPN-4 was localized to the posterior blastomeres, including the germline precursors, and became undetectable by mid-embryogenesis. Primary Identifier  Expr2552
Remark  A granular staining pattern was found for SPN-4 in the germline blastomeres. This pattern exactly matched the granular staining pattern for POS-1, suggesting that these structures corresponded to P granules. Indeed, the granular patterns matched exactly those observed with a P-granule-specific antibody, indicating that SPN-4 is a component of P granules. The specificity of the antibody was confirmed by the fact that SPN-4 was detected neither in the deletion mutant tm291 nor in spn-4(RNAi) embryos. The distribution of SPN-4 overlapped with that of POS-1 in the posterior blastomeres.
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10 Anatomy Terms

Definition Name Synonym Primary Identifier
a female haploid germ cell. oocyte ovum WBbt:0006797
Single cell zygote P0 lineage name: Z WBbt:0004422
embryonic founder cell Psub1 lineage name: P0.p WBbt:0006874
embryonic founder cell Psub2 lineage name: P0.pp WBbt:0006873
embryonic cell EMS   WBbt:0006876
embryonic cell ABa   WBbt:0005906
embryonic cell ABp   WBbt:0005994
Embryonic founder cell AB AB blastomere WBbt:0004015
Germ line precursor cell Z3 lineage name: P4.a WBbt:0004575
Germ line precursor cell Z2 lineage name: P4.p WBbt:0004576

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00004984 spn-4 ZC404.8 Caenorhabditis elegans

2 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation. blastula embryo Ce WBls:0000005