WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  pCZ178 and pCZ179 were expressed in similar sets of cells, however, GFP expression from pCZ178 was much weaker than from pCZ179 in most cells. CND-1::GFP expression was first detectable in four descendants of the AB lineage of 14 cell embryos. AB derived neuroblasts give rise to most of the C. elegans nervous system. By the 24-cell stage, approximately 75 minutes postfertilization, CND-1::GFP was found in 15 AB derived blastomeres. CND-1::GFP expression was observed in numerous unidentified nuclei throughout gastrulation and epidermal enclosure. By early comma stage, approximately 360 minutes postfertilization, CND-1::GFP was found in many postmitotic neurons in the head and in the ventral cord. The expression of CND-1::GFP in the ventral cord neurons was maintained until hatching, but disappeared completely by the end of the first larval stage. CND-1::GFP was not observed in postembryonically derived motor neurons. To determine whether cnd-1 was expressed in non-neuronal cells of the AB lineage, embryos carrying cnd-1::GFP transgenes were double labeled with anti-GFP and anti-LIN-26 antibodies. In all embryonic stages examined the expression of CND-1::GFP and LIN-26 did not overlap. To confirm the identity of specific AB-derived blastomeres during later stage embryogenesis, embryos carrying CND-1::GFP transgenes were double labeled with anti-GFP and anti-UNC-86 antibodies. CND-1::GFP and UNC-86 are primarily expressed in different subsets of mitotic and postmitotic neurons throughout embryogenesis. At 230 minutes postfertilization, UNC-86 is expressed in ABplaaaaaa, ABarpapaaa, ABplapaaaa, ABprapaaaa, ABarppaaap, and ABarpppapp blastomeres (Finney and Ruvkun, 1990). At the same stage, CND-1::GFP was found in a non-overlapping set of neuroblasts derived from the ABplppap and ABprppap, including ABplppaap, ABplppapp, ABplpppaa, ABprppapa, ABprppaap, ABprppapp and ABprpppaa, which give rise to the embryonic ventral cord motor neurons. In summary, reporter transgene analysis reveals that CND-1::GFP is expressed in both mitotically active neuroblasts throughout embryogenesis, and in subsets of postmitotic neurons including the ventral cord motor neurons. Primary Identifier  Expr954
Remark  Transgenic lines containing pCZ178 rescued both the uncoordination and cellular defects of the ventral cord motor neurons of cnd-1(ju29) mutants. To minimize misrepresentation caused by the mosaicism of the extrachromosomal arrays, authors collected data from three independent transgenic lines containing pCZ179 or pCZ178 respectively. Subcellular Localization  Transgenic lines containing pCZ178 show that GFP was localized to the nucleus.
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34 Anatomy Terms

Definition Name Synonym Primary Identifier
neuron with cell body associated with the ventral nerve cord. ventral cord neuron ventral cord motoneuron WBbt:0005300
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
A blast cell from which a neuron develops. neuroblast   WBbt:0008594
embryonic cell ABarp   WBbt:0006653
embryonic cell ABprp   WBbt:0006451
embryonic cell ABplp   WBbt:0006279
embryonic cell ABalp   WBbt:0005865
embryonic cell ABplap   WBbt:0006096
embryonic cell ABalap   WBbt:0006101
embryonic cell ABala   WBbt:0006707
embryonic cell ABarap   WBbt:0005903
embryonic cell ABprpa   WBbt:0005915
embryonic cell ABplpa   WBbt:0005950
embryonic cell ABpla   WBbt:0006341
embryonic cell ABprap   WBbt:0005922
embryonic cell ABprpp   WBbt:0006347
embryonic cell ABaraa   WBbt:0006031
embryonic cell ABplpp   WBbt:0006353
embryonic cell ABpraa   WBbt:0006007
embryonic cell ABara   WBbt:0006162
embryonic cell ABalpa   WBbt:0006083
embryonic cell ABpra   WBbt:0006581
embryonic cell ABplaa   WBbt:0006121
embryonic cell ABarpa   WBbt:0006008
embryonic cell ABalaa   WBbt:0006039
embryonic cell ABarpp   WBbt:0006046
embryonic cell ABalpp   WBbt:0006486
embryonic cell ABprppapa   WBbt:0006092
embryonic cell ABprppapp   WBbt:0005929
embryonic cell ABplppapp   WBbt:0006356

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000561 cnd-1 C34E10.7 Caenorhabditis elegans

1 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  All C. elegans development stages, including embryo, larva and adult stage. all stages Ce WBls:0000002