WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  DAF-18::GFP expression was observed in many tissues during all larval stages, including the developing vulva, the uterus, the ventral nerve cord and the distal tip cells. In particular, equal levels of DAF-18::GFP expression were detected in the six VPCs of L2 larvae, and expression persisted in the descendants of the induced VPCs until the Pn.pxxx stage. Besides the vulval cells, the DAF-18::GFP reporter was also expressed at the L3 to L4 larval stages in several cells of the uterus, which is part of the somatic gonad. Primary Identifier  Expr10551
Subcellular Localization  The sub-cellular localization of DAF-18::GFP changed over the course of vulval development. In the VPCs of L2 larvae prior to and during induction (Pn.p stage), DAF-18::GFP was predominantly localized in the cytoplasm and the nucleus. However, at the subsequent stages (Pn.px to Pn.pxx stages), DAF- 18::GFP became increasingly localized to the plasma membrane of the vulval cells. Plasma membrane staining peaked at the 'Christmas tree' (Pn.pxxx) stage, when almost all the protein appeared to be localized to the membranes and nuclear staining was reduced to very low levels.
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5 Anatomy Terms

Definition Name Synonym Primary Identifier
female genital. vulva   WBbt:0006748
somatic cell that situates at the tip of a gonad arm. distal tip cell DTC WBbt:0006865
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring. ventral nerve cord ventral cord WBbt:0005829
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
cell that has the potential to form structural part of a vulva. vulval precursor cell VPC WBbt:0007809

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000913 daf-18 T07A9.6 Caenorhabditis elegans

4 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024