WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  To investigate whether shorter twk-40 promoters might exhibit neuronal specificity, we examined an upstream portion (Ptwk-40L, 2.5 kb, upstream of twk-40b first exon) and a downstream portion (Ptwk-40R, 2.8 kb, upstream of twk-40a first exon, including the first exon of twk-40c) of the Ptwk-40F promoter. A Ptwk-40L::GFP reporter and a Ptwk-40R:: mCherry reporter both labeled C. elegans head neurons, the ventral nerve cord, and tail neurons. However, it appeared that Ptwk-40R::mCherry also labeled motor neurons while Ptwk-40L::GFP did not. We next crossed the two transgenes into same animals. In these animals, we failed to identify head neurons obviously co-labeled by the two reporters. Interestingly, an unidentified tail neuron at the position of DVA/DVB/DVC was co-labeled. Consistently, multiple motor neurons were labeled by Ptwk-40R::mCherry but not by Ptwk- 40L::GFP. We next generated transgenic animals co-expressing Plim-6int4::GFP with Ptwk-40L::mCherry or Ptwk-40R::mCherry. Here we found that Ptwk-40L::mCherry labeled DVA/DVC but not DVB, while Ptwk-40R::mCherry labeled DVB/DVC but not DVA. Primary Identifier  Expr16593
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6 Anatomy Terms

Definition Name Synonym Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring. ventral nerve cord ventral cord WBbt:0005829
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
neuron with its cell body situated in the tail, posterior to rectum. tail neuron   WBbt:0006759
neuron making synapse on an effector (muscle, gland). motor neuron motoneuron WBbt:0005409
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, inervates rectal muscles. DVB lineage name: K.p WBbt:0004822
Neuron class of one ring interneuron, cell body in dorsorectal ganglion. DVC lineage name: Caapaa WBbt:0004821

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00006691 twk-40 T28A8.1 Caenorhabditis elegans

0 Life Stages