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WSWS298

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Expression Pattern :

Pattern  During larval stages, ceh-43::gfp diminishes and is almost absent in adults. In newly hatched larvae, we identified the binucleate dorsal hyp3 and ventral hyp4 cells, the CAN neurons, and the PVQ neurons. Sheath and/or socket cells of several other anterior sensilla are stained as well, identified from their morphology and their position around the pharynx procorpus. In 3-fold stage embryos that move around in the eggshell, the mouth opening can occasionally be seen from above; at the tip of the head, a small ring encircles the mouth opening. It most likely belongs to a small cylindrical hypodermal syncytium such as hyp4 or hyp3, or other small hypodermal cells that connect the buccal cavity to the mouth. All the identified and suspected cells belong to the AB lineage. ceh-43::gfp expression starts around gastrulation in several superficial cells. By following cell divisions, almost all fifth generation descendants of the AB blastomere were identified as GFP positive. Shortly before morphogenesis, GFP expressing cells lie anteriorly, laterally, and anterodorsally at the surface of the embryo. These positions are characteristic for clones of AB descendants. Not all the progeny of the AB blastomere express GFP. At 1.5-fold egg length, most embryonic cell divisions have occurred. At that time, GFP expression is strongest in cells surrounding an indentation at the anterior end, the anterior sensory depression, as well as in two superficial bilateral cells and one ventral cell in the tail. Among these cells are most likely the cells identified in larvae or their precursors. GFP expression is weaker in lateral head regions, the position of future nerve ring neurons. Primary Identifier  Expr1681
Remark  During all developmental stages, ceh-43::gfp expression resembles the antibody staining pattern published by Panganiban et al. (1997). Therefore, both methods are likely to reflect the natural ceh-43 expression pattern. However, gfp expression is visible generally in fewer cells and is absent from most nerve ring neurons. Therefore, the reporter may not show the complete expression pattern. The short pDllB promoter drives expression only in the main body syncytium, hyp7, where pDllA is not expressed. Authors believe that this expression may not reflect the natural ceh-43::gfp expression, because it is not consistent with the antibody staining from Panganiban et al. (1997). Reporter gene fusion type not specified.
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23 Anatomy Terms

Definition Name Synonym Primary Identifier
a structural (glial) cell which forms an specialized environment surrounding the sensory ending(s) of one or more neurons; sometimes accompanied by a more distal socket cell. In early development of the sensory nerves and of the nerve ring some sheath cells (cephalics and labials) may also provide a substrate for axon guidance neuronal sheath cell pocket cell WBbt:0005811
Cylindrical hypodermal syncytium in head hyp3 lineage name: ABplaapaaaa WBbt:0004690
Cylindrical hypodermal syncytium in head hyp4 lineage name: ABarpapapa WBbt:0004687
Interneuron, projects along ventral cord to ring PVQL lineage name: ABplapppaaa WBbt:0004076
Interneuron, projects along ventral cord to ring PVQR lineage name: ABprapppaaa WBbt:0004074
Process runs along excretory canal, no synapses, essential for survival CANL lineage name: ABalapaaapa WBbt:0004949
Process runs along excretory canal, no synapses, essential for survival CANR lineage name: ABalappappa WBbt:0004947
embryonic cell ABplap   WBbt:0006096
embryonic cell ABalap   WBbt:0006101
embryonic cell ABarap   WBbt:0005903
embryonic cell ABprpa   WBbt:0005915
embryonic cell ABplpa   WBbt:0005950
embryonic cell ABprap   WBbt:0005922
embryonic cell ABprpp   WBbt:0006347
embryonic cell ABaraa   WBbt:0006031
embryonic cell ABplpp   WBbt:0006353
embryonic cell ABpraa   WBbt:0006007
embryonic cell ABalpa   WBbt:0006083
embryonic cell ABplaa   WBbt:0006121
embryonic cell ABarpa   WBbt:0006008
embryonic cell ABalaa   WBbt:0006039
embryonic cell ABarpp   WBbt:0006046
embryonic cell ABalpp   WBbt:0006486

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000463 ceh-43 C28A5.4 Caenorhabditis elegans

5 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015
  The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. enclosing embryo Ce WBls:0000013
  The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation. gastrulating embryo Ce WBls:0000010
  A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death. postembryonic Ce WBls:0000022