WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  The gene T03G6.3, chosen based on data from the C. elegans Expression Pattern Consortium, gave GFP expression in bodywall muscle cells of embryos at the three-fold stage of elongation and continued expression within these cells through all larval stages into adulthood, with further expression in the vulval muscles. Within embryos there was not any discernable subcellular localisation of the fluorescence but from the larval stages onwards, the fusion protein localised to distinct subcellular structures within the bodywall muscles. The distribution changed at different focal planes of the muscle, puncta being visible upon the outer regions of the cell whilst deeper within the cell the distribution appeared more uniform, interrupted with irregular areas without fluorescence. The arrangement of the puncta was consistent with the T03G6.3::GFP being localised to dense bodies. The puncta sometimes appeared to be linked with slightly thinner lines acting as connections suggesting the protein was localised all along the centre of the I-bands, but enriched in the dense bodies. There did not seem to be any fusion protein in the nucleus. The distribution of the fusion protein appeared uniform within the vulval muscles. Fluorescence in all stages was very faint in both the integrated lines obtained and all three non-integrated lines. Co-localisation experiments were performed for T03G6.3 and C46G7.2 fusion proteins to confirm the localisation sites within the sarcomere. T03G6.3 localises to the centre of the I-bands and the presence in puncta suggests that it is enriched in the dense bodies. Primary Identifier  Expr13612
Remark  Strain UL2448
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2 Anatomy Terms

Definition Name Synonym Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00020195 T03G6.3 T03G6.3 Caenorhabditis elegans

4 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood. larva Ce WBls:0000023
  The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. 3-fold embryo Ce WBls:0000020