WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  Expression was first observed in the developing embryo at the 1.5- to 2-fold stage, also observed in larvae and adults. Expression observed in body wall muscle, anal depressor muscle and vulval muscles. Primary Identifier  Expr2577
Remark  Five independent lines in a wild-type background showed the same pattern of expression, although the level of expression varied somewhat between transgenic lines. Significantly, the same UNC-98::GFP was used to create two independent lines in an unc-98(su130) background, and both lines are fully rescued with respect to the muscle phenotype. In addition, the UNC-98::GFP showed that same pattern of expression and intracellular localization in both wild-type and unc-98(su130) backgrounds. Subcellular Localization  Expression was first observed in the developing embryo at the 1.5- to 2-fold stage, in which the UNC-98-GFP protein seemed localized to filamentous tracts, which generally follow myosin heavy chain A antibody staining, and therefore are interpreted to be located in myofibrils. Additionally, expression is seen in undefined puncta located along the filamentous tracts. In larvae and adults, expression was seen in body wall muscle, and in addition, anal depressor muscle and vulval muscles. Within adult body wall muscle, the UNC-98-GFP fusion localizes to dense bodies and M-lines. Within these muscle cells, prominent localization of the UNC-98-GFP in the nucleus was also seen. The UNC-98-GFP colocalizes with the DNA-binding dye DAPI. The signal is excluded from what seems to be the nucleolus and the UNC-98-GFP signal is discontinuous. This nuclear localization is consistent with the presence of the NLS sequences noted above. During development, the nuclear localization of UNC-98::GFP begins at the late L2 to early L3 larval stages.
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3 Anatomy Terms

Definition Name Synonym Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents. anal depressor muscle dilator muscle WBbt:0004292

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00006827 unc-98 F08C6.7 Caenorhabditis elegans

9 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. 2-fold embryo Ce WBls:0000019
  The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. 3-fold embryo Ce WBls:0000020
  The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo. 1.5-fold embryo Ce WBls:0000018