WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Primary Identifier  Expr3230 Subcellular Localization  In the bean-stage preelongation embryo of a wild-type strain, EFF-1::GFP was initially seen in a punctate but unpolarized pattern in cells of the ventral and dorsal hypodermis. As expression increased, a uniform cytoplasmic fluorescence appeared surrounding the bright punctate bodies (possibly endoplasmic reticulum or Golgi apparatus). Isolated EFF-1::GFP-expressing cells, with no brightly expressing neighbors, retained an unpolarized distribution of fluorescence. In contrast, EFF-1::GFP became discretely and rapidly localized to contacts between pairs of brightly expressing fusion partner cells. Two types of EFF-1::GFP-enriched contacts could be observed: novel contacts between cells already expressing EFF-1::GFP and preexisting contacts between neighbors in which EFF-1::GFP expression arose after the contact was made. In the first case (novel contacts), two ventral cells expressing EFF-1::GFP migrated toward each other to meet at the ventral midline. Although fluorescence was not plasma-membrane associated during cell migration, a brightly fluorescent junction formed within minutes of initial contact between the two cells. This localization grew in intensity over the next 20 min. In the second case (preexisting contacts), two neighboring dorsal cells began to express EFF-1::GFP. At the interface between these cells, localized EFF-1::GFP became brighter in coincidence with their overall expression of EFF-1::GFP. In both cases (novel and preexisting contacts), the increase in localized fluorescence was approximately linear with time. Furthermore, the contact-localized fluorescence achieved intensities far exceeding the mere sum of brightness of two separate cell membranes, indicating continuous targeted accumulation at the interface. In dorsal hypodermal cells, EFF-1::GFP accumulated along the apical edge of the cell-cell interface, coincident with the intercellular junctions and the known origin of the fusion aperture between cells. Interestingly, the membrane accumulation of EFF-1::GFP persisted, even after the contents of two cells were mixed, indicating that cell fusion was underway or complete. In occasional recordings of ventral cells, the localized EFF-1::GFP appeared to be pushed aside, presumably by the widening cell-fusion aperture, as the cells fused.
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1 Anatomy Terms

Definition Name Synonym Primary Identifier
Epidermal layer. hypodermis epidermis WBbt:0005733

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001159 eff-1 C26D10.5 Caenorhabditis elegans

7 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015