| Pattern | In contrast to adult male germlines, no FOG-1 was detected in adult hermaphrodites. Therefore, FOG-1 expression is sexually dimorphic in adults. FOG-1 is graded in spermatogenic germlines: FOG-1 is low or undetectable in proliferating cells but abundant in cells entering the meiotic cell cycle and destined for spermatogenesis. By contrast, FOG-1 is not detected in oogenic germlines. In wild-type animals, FOG-1 protein was observed in the germline and was predominantly cytoplasmic. In L2s, FOG-1 became detectable, but staining was faint. In L3s, the level of FOG-1 remained low distally in proliferating germ cells, but FOG-1 was abundant more proximally in germ cells that had entered meiosis and were destined for spermatogenesis. Temperature shift experiments with a fog-1(ts) allele showed that FOG-1 specifies spermatogenesis in L3 when germ cells enter meiosis, consistent with the idea that the abundant FOG-1 in early meiotic germ cells is specifying the sperm fate. In adult male germlines, FOG-1 was spatially graded: FOG-1 was either not detected or barely visible in the distal half of the mitotic region, became detectable in the proximal half of the mitotic region where some germ cells have entered pre-meiotic S phase, intensified in the transition zone and remained high in distal pachytene germ cells; no FOG-1 was detected in more proximal pachytene cells. | Primary Identifier | Expr3514 |
| Remark | Gene_regulation: In fbf-1 fbf-2 mutants, FOG-1 levels were elevated compared with wild-type. In both L2 and L3, FOG-1 was easily detectable or abundant in all fbf-1 fbf-2 germ cells. Therefore, FBF repression is required for maintaining low FOG-1 in L2 germlines and for establishing the FOG-1 spatial gradient in L3 germlines. To assess FOG-1 in germlines that possess proliferative cells throughout the entire tissue, germlines that are tumorous were stained in the presence and absence of FBF. The quantity of FOG-1 protein was low in gld-3 nos-3 tumorous germlines, but consistently higher in fbf-1 fbf-2 gld-3 nos-3 tumorous germlines. The FBF represses fog-1 expression in vivo and that FBF is required for establishing the temporal and spatial pattern of FOG-1 expression. This adult male pattern of fog-1 expression was confirmed by in situ hybridization using an antisense probe to detect fog-1 mRNA; no RNA was seen with a sense probe. Germlines dissected from fog-1(q250) mutant males had no detectable FOG-1 protein, consistent with its being a null allele and demonstrating specificity of the antibody. | Subcellular Localization | cytoplasmic |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes. | germ line | germline | WBbt:0005784 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001481 | fog-1 | Y54E10A.4 | Caenorhabditis elegans |
Powered by
