WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Primary Identifier  Expr11270 Subcellular Localization  A reticular pattern was observed for both RSD-2A and RSD-2B isoforms in intestinal cells; however, the smaller RSD-2B protein displayed an additional, more diffuse pattern of staining in the cytoplasm. In double-labeling experiments using anti-GFP and anticalreticulin antibodies, we observed colocalization of fluorescent signals in some animals(50-80%). Calreticulin harbors a KDEL localization motif and resides in the lumen of the endoplasmic reticulum. The localization pattern of GFP in wild type was reticular and similar to the subcellular pattern obtained using fixed tissues. While the cytoplasmic compartment contained most of the RSD-2TGFP protein, additional staining for bothRSD-2A and RSD-2B was observed in a subnuclear compartment. We had previously observed nucleolar staining in germline nuclei, oocytes, fertilized embryos, and intestinal cells in fixed animals using anti-GFP antibodies; however, the staining intensity was often weak and was not observed in all immunolocalization experiments. The integrated transgene expressing the RSD-2ATGFP reporter also accumulated GFP fluorescence in the nuclei of live animals.
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1 Anatomy Terms

Definition Name Synonym Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00004681 rsd-2 F52G2.2 Caenorhabditis elegans

0 Life Stages