WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  In L1 larvae, PDI-3 localized in many tissues with predominant staining found in the hypodermal and the gut tissues. It can be noted that the pharynx, gut lumen, and body cavity were not stained with either antibody tested. Wild type embryos and larvae were probed with a PDI-3-specific polyclonal antibody in combination with a DPY-7-specific monoclonal antibody. DPY-7 is a cuticle collagen that is expressed solely in the hypodermis and is localized in the cuticular ECM from the elongated embryo stage onwards. In the 2-fold embryo (~500 min after fertilization), PDI-3 staining was observed in most tissues including the gut and the hypodermis. Conversely, DPY-7 localized exclusively in the hypodermal cells. Primary Identifier  Expr2350
Subcellular Localization  Gut nuclei did not stain and appeared as dark patches among the PDI-3-labeled gut tissue. DPY-7 antibody was localized solely to the annular furrows of the hypodermally derived cuticle.
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2 Anatomy Terms

Definition Name Synonym Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
Epidermal layer. hypodermis epidermis WBbt:0005733

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003964 pdi-3 H06O01.1 Caenorhabditis elegans

5 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003