3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003183 | mei-1 | T01G9.5 | Caenorhabditis elegans |
| WBGene00006528 | tba-1 | F26E4.8 | Caenorhabditis elegans |
| WBGene00006540 | tbg-1 | F58A4.8 | Caenorhabditis elegans |
| Primary Identifier | Expr8293 | Remark | Picture: Fig. 7, Fig. 5, and Fig. S2. |
| Subcellular Localization | Meiotic spindle assembly initiates at nuclear envelope breakdown, when a diffuse cloud of microtubules fills the volume of the perforated nucleus. These microtubules then organize into a much smaller assembly of dense microtubule bundles by the time the oocyte ovulates into the spermatheca. By the time the embryo exits the spermatheca and enters the uterus, a bipolar, metaphase-length spindle is present within a vesicle-free zone visible by differential interference contrast. Before NEBD, GFP::TBG-1 became progressively more concentrated on the nuclear envelope as oocyte maturation progressed. At nuclear envelope breakdown, GFP::TBG-1 fluorescence entered the nuclear region, forming a diffuse cloud that might indicate association with either microtubules or vesicles derived from the nuclear envelope. This large, diffuse area of gamma-tubulin enrichment did not contract in diameter nor increase in local intensity, as did the alpha-tubulin. By the time the embryo exited the spermatheca into the uterus, no discrete localization of GFP::TBG-1 was observed in the vesicle-free zone that contains the metaphase I spindle. GFP::MEI-1 was also distributed throughout the volume of the perforated nucleus immediately after nuclear envelope breakdown. GFP::MEI-1 differed from GFP::TBG-1 in that it became highly concentrated on chromosomes and spindle poles during spindle assembly and remained concentrated on these structures throughout meiosis. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00003183 | mei-1 | T01G9.5 | Caenorhabditis elegans |
| WBGene00006528 | tba-1 | F26E4.8 | Caenorhabditis elegans |
| WBGene00006540 | tbg-1 | F58A4.8 | Caenorhabditis elegans |
Powered by
