1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00010272 | amph-1 | F58G6.1 | Caenorhabditis elegans |
| Primary Identifier | Expr8855 | Remark | Picture: Fig 2, Fig S2, S3. |
| Subcellular Localization | Endogenous AMPH-1 colocalizes extensively with endogenous RME-1 on tubulovesicular basolateral recycling endosomes, and on more weakly labelled sub-apical structures of the worm intestine, possibly the apical recycling endosomes. Although there was significant overlap of RME-1 and AMPH-1 labelling on the recycling endosome, the two proteins did not label the endosome identically. Endogenous AMPH-1 also colocalized with basolateral GFP-tagged SDPN-1 (a C. elegans homologue of synaptic dynamin binding protein, also known as pacsin), another marker for the C. elegans recycling endosome. Anti-AMPH-1 staining failed to colocalize with markers for clathrin-coated pits (GFP-tagged CHC-1/clathrin), early endosomes (GFPRAB-5), late endosomes (GFPRAB-7) or the Golgi (mannosidaseGFP), indicating that AMPH-1 is specifically enriched on recycling endosomes. Extensive colocalization of mCherryRME-1 and AMPH-1GFP was also observed in living transgenic animals, supporting the data derived from fixed tissue. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00010272 | amph-1 | F58G6.1 | Caenorhabditis elegans |
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