| Primary Identifier | Expr16119 | Subcellular Localization | To investigate the expression and subcellular localization of GRDN-1 in A/PQR, we expressed the functional GFP-tagged full-length GRDN-1a isoform (henceforth referred to as GRDN-1::GFP) under its endogenous regulatory sequences (Nechipurenko et al., 2016). GRDN-1::GFP was expressed in A/PQR and localized to the cilia base in both neurons in adult hermaphrodites. The dynein light intermediate chain protein XBX-1 localizes to the basal body and undergoes bidirectional movement along the ciliary axoneme (Schafer et al., 2003). The GRDN-1::GFP signal overlapped with the proximal-most region of the XBX-1::tagRFP signal in PQR, suggesting that GRDN-1 localizes to the basal body in PQR. GRDN-1 also associates with the basal body in the distal segment of the outgrowing AQR dendrite. |
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| Neuron class of one sensory neuron, basal body, not part of a sensillum, projects into preanal gangion. | PQR | lineage name: QL.ap | WBbt:0004096 |
| Neuron class of one sensory neuron, basal body; not part of a sensillum, projects into ring. | AQR | lineage name: QR.ap | WBbt:0003927 |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00013082 | grdn-1 | Y51A2D.15 | Caenorhabditis elegans |
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