WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  the CRISPR line showed MLCK-1 expression in the pharynx, uterus, spermatheca, and sp-ut valve. MLCK-1 is seen throughout the spermatheca, including the distal neck, bag, and valve cells. By mid L4, faint MLCK-1 expression is evident in the cells of the developing spermatheca. Expression levels peak in adulthood. During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. In adults, MLCK-1 is localized both apically and basally in the spermatheca. As expected, we observed thick bundles of actin at the outer, basal surface with a thinner actin network visible at the apical surface. MLCK-1 fluorescence intensity is brightest at the apical boundaries. However, some MLCK-1 is also localized to the basal cell surface, where it partially colocalizes with the contractile actomyosin bundles. Primary Identifier  Expr13846
Reporter Gene  CRISPR line COP1510 mlck-1::degron::mKate2 endogenously tagged with GFP strain, was generated by Nemametrix. Subcellular Localization  During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. In adults, MLCK-1 is localized both apically and basally in the spermatheca.
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4 Anatomy Terms

Definition Name Synonym Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
A single syncytium cell of four nuclei makes a valve, connecting spermatheca and uterus of hermaphrodite gonad. spermathecal-uterine valve cell sp-ut cell WBbt:0008217

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00013869 mlck-1 ZC373.4 Caenorhabditis elegans

0 Life Stages