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WSWS298

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Expression Pattern :

Pattern	NID-1 was first detected in embryos at the beginning of morphogenesis (lima stage) localized to body wall muscle cells. As embryos elongate strong NID-1 staining is seen around body wall muscle cells and diffuse stain begins to accumulate on the surfaces of the pharyngeal and intestinal primordia. Once the embryo has elongated to the 2-fold stage, NID-1 has localized to the basal face of the body wall muscles and shows strong accumulation on the surfaces of the pharyngeal, intestinal, and gonad primordia. In three- and fourfold stage embryos, NID-1 accumulates to higher levels and remains localized under the four body wall muscle quadrants and on the surfaces of the pharynx, intestine, and gonad. In L1 larvae the intensity of staining of body wall muscle, pharynx, and intestine appears reduced, and strong staining associated with the nerve ring becomes apparent. This pattern continues through the L2 and L3 larval stages, with the addition of stronger staining of the distal tip cells as they lead the growth of the gonad. In late L3 to L4 stage larvae particularly strong NID-1 accumulation is seen associated with the distal tip cells and the developing somatic structures of the gonad, the spermatheca, uterus, and vulva. Under the body wall muscles of larval and adult stage animals NID-1 is organized as punctate lines. These lines follow the rows of dense bodies within the muscle cells. NID-1 also accumulates strongly at the outer edges of the muscle quadrants and more weakly at the boundaries between muscle cells within each quadrant. Less organized NID-1 staining is seen in the regions between the body wall muscle quadrants, presumably associated with the epidermal basement membranes in these regions. NID-1 accumulates along the four sublateral nerves that run beneath the center of each muscle quadrant. The sublateral nerves extend along dorso- and ventrolateral tracts from the nerve ring in the anterior of the animal to near the middle of the animal where they turn further lateral to positions coincident with the lateral edges of the body wall muscle quadrants. NID-1 accumulation on these nerves is seen in larval and adult animals and is similar in intensity to the staining at the edges of the body wall muscle quadrants. Staining along the edges of the body wall muscle quadrants appears to be associated with the muscle edges rather than the nerves in these regions because the staining closely follows the edge of the muscles and it does not display the left/right asymmetry expected for the ventral and dorsal nerve cords. Less organized NID-1 staining is also present in the regions between body wall muscle quadrants.	Primary Identifier	Expr1074
Remark	lima stage (author) = bean embryo (wjc).	Subcellular Localization	membranes

16 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
adult vulval toroid cell, F, formed by fusion of precursors of lineage names P6.papl, P6.papr, P6.ppal and P6.ppar.	vulF		WBbt:0006768
adult vulval toroid cell, E, formed by fusion of precursors of lineage names P6.paal, P6.paar, P6.pppl and P6.pppr.	vulE		WBbt:0006767
organ producing either sperm or ova.	gonad		WBbt:0005175
adult vulval toroid cell, C, formed by fusion of precursors of lineage names P5.ppal, P5.ppar, P7.papl and P7.papr.	vulC		WBbt:0006765
adult vulval toroid cell, B1, formed by fusion of precursors of lineage names P5.papa and P7.ppap.	vulB1		WBbt:0006763
adult vulval toroid cell, B2.	vulB2		WBbt:0006764
adult vulval toroid cell, D, formed by fusion of precursors of lineage names P5.ppp and P7.paa.	vulD		WBbt:0006766
adult vulval toroid cell, A, formed by fusion of precursors of lineage names P5.paaa, P5.paap, P7.pppa and P7.pppp	vulA		WBbt:0006762

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003738	nid-1	F54F3.1	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death.		postembryonic Ce	WBls:0000022

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