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WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  In embryos, GFP expression was first detected at the 2-fold stage. Sometimes eight cells in the anterior half of the body expressed GFP. At the 3-fold stage, several cells around the pharynx expressed GFP in most embryos. About half of the embryos also expressed GFP weakly in the pharynx. At L1, GFP was detected in a subset of neurons. About half of the larvae also expressed GFP in the pharynx; expression in the terminal bulb was the most intense. At the adult stage, GFP was detected in the pharyngeal muscles, m3, m4, and m6. In addition, GFP was detected in a subset of neurons: IL1, OLQ, BAG, and ALN, several circumpharyngeal cells, and coelomocytes. Very faint GFP fluorescence was detected in the pharyngeal neurons including I4 and I5 in L1 larvae. Primary Identifier  Expr976
Remark  Reporter gene fusion type not specified. The antibody staining showed EAT-20 expression in the seam cells and hypodermal cells, which is inconsistent with the absence of GFP expression in those cells in the promoter trap lines, suggesting that p5E5 may not contain all the cis-regulatory elements necessary for the expression of the eat-20 gene. Reporter Gene  GFP expression was analyzed in ncIs1 hermaphrodites carrying p5E5 as a stable integrated transgenic array. Though p5E5 contains a 2-kb genomic fragment derived from LGV at the 5' side in addition to the fragment corresponding to the eat-20 gene, it is confirmed that an extrachromosomal array of p5E5 and that of p5E5del, in which the 2-kb fragment was deleted, gave the same GFP expression pattern. Thus, the fragment at the 5' part of the insert had no effects on the expression of GFP. Neurons expressing GFP were first identified in L1 larvae.
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21 Anatomy Terms

Definition Name Synonym Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
Neuron, ciliated ending in head, no supporting cells, associated with ILso BAGR lineage name: ABarappppap WBbt:0003823
Neuron, ciliated ending in head, no supporting cells, associated with ILso BAGL lineage name: ABalppappap WBbt:0003824
Neuron class of one pharyngeal interneuron, posterior sensory. I5 neuron I5 WBbt:0004740
Inner labial neuron IL1VR lineage name: ABarapppppaa WBbt:0004547
Inner labial neuron IL1DR lineage name: ABalappppaaa WBbt:0004737
Inner labial neuron IL1L lineage name: ABalapaappaa WBbt:0004553
Inner labial neuron IL1R lineage name: ABalaappppaa WBbt:0004551
Inner labial neuron IL1VL lineage name: ABalppapppaa WBbt:0004549
Inner labial neuron IL1DL lineage name: ABalapappaaa WBbt:0004738
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles. coelomocyte   WBbt:0005751
fourth pharyngeal muscle cell layer pm4 M4 WBbt:0003739
third pharyngeal muscle cell layer pm3 M3 WBbt:0003740
Quadrant outer labial neuron OLQVL lineage name: ABplpaaappaa WBbt:0004437
Quadrant outer labial neuron OLQVR lineage name: ABprpaaappaa WBbt:0004431
Quadrant outer labial neuron OLQDL lineage name: ABalapapapaa WBbt:0004439
Quadrant outer labial neuron OLQDR lineage name: ABalapppapaa WBbt:0004438
sixth pharyngeal muscle cell layer pm6 m6 WBbt:0003724
Neuron associated with ALM ALNL lineage name: ABplapappppap WBbt:0003939
Neuron associated with ALM ALNR lineage name: ABprapappppap WBbt:0003937
Neuron class of one pharyngeal interneuron. I4 neuron I4 WBbt:0004741

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001148 eat-20 H30A04.1 Caenorhabditis elegans

4 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. 2-fold embryo Ce WBls:0000019