WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  Examination of transgenic animals bearing the transcriptional fusion revealed that pan- 1prom::GFP is expressed in most somatic tissues. Expression was observed in all developmental stages from embryos undergoing morphogenesis to gravid adults. pan- 1prom::GFP exhibited strong expression in body wall muscle, vulva, somatic gonad and pharynx. Expression was also observed in the nerve ring, hypodermis, and rectal epithelia. Expression in the intestine was typically not observed or very weak. With respect to spatial expression, the pattern of pan-1FL::GFP was the same as pan-1prom::GFP with some notable exceptions. First, PAN- 1FL::GFP exhibited differences in expression intensity depending on the developmental point within the intermolt period. PAN-1FL::GFP was substantially qualitatively brighter at the mid to late intervals of the intermolt. Early in the intermolt bright expression of PAN- 1FL::GFP was typically only observed in the pharynx. Expression was also weak in the adult with the pharynx being the predominant expressing tissue. The other difference with respect to expression is that PAN-1FL::GFP was observed in the intestine, especially when expression was bright towards the end of the molt. Primary Identifier  Expr11020
Subcellular Localization  PAN-1FL::GFP exhibited a punctate localization in expressing cells. In the vulva and somatic gonad epithelia (spermatheca and uterus), these punctae were concentrated on the apical (luminal) surface of these cells. During embryonic pharyngeal development, PAN-1FL::GFP was found in the apical pharyngeal epithelium, as indicated by co-staining with the monoclonal antibody MH27 which recognizes the adherens junctions of epithelial cells. The localization in apical regions of epithelial cells is consistent with membrane association of the PAN-1B isoform. Additionally, immunolocalization data for PAN-1 indicate membrane association in intestinal cells. Cytoplasmic expression of GFP is also observed (likely corresponding to PAN-1A or PAN-1C); especially in the pharynx but also in other cell types. However, apparent cytoplasmic expression was not consistently observed indicating that some cell types may only express cytoplasmic isoforms of PAN-1 at certain developmental times.
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11 Anatomy Terms

Definition Name Synonym Primary Identifier
female genital. vulva   WBbt:0006748
an accordion-like tube that contains sperm and is the site of oocyte fertilization. spermatheca   WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
Epidermal layer. hypodermis epidermis WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
The organ in which the eggs are developed and protected until laid. uterus   WBbt:0006760
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail seam cell lateral hypodermis WBbt:0005753
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes. nerve ring circumpharyngeal nerve ring WBbt:0006749
The components of the gonad that are separate from the germline proper. In hermaphrodite, these include five tissues which are all derived from the somatic primordium : the distal tip cells, the gonadal sheath, the spermatheca, the spermatheca-uterine valve (sp-ut) and the uterus. somatic gonad somatic germline WBbt:0005785
These cells include: B, F, Y, U, K' and K. All rectal epithelial cells contain secretory membrane stacks along this region facing the lumenal cuticle and produce some portion of the cuticular lining of the rectum. These are interfacial cells that connect the alimentary system to the main body syncytium. rectal epithelial cell rectal epithelium WBbt:0008182

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003915 pan-1 M88.6 Caenorhabditis elegans

1 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  All C. elegans development stages, including embryo, larva and adult stage. all stages Ce WBls:0000002