WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern  When transgenic animals carrying pTG96 on an extrachromosomal array (kuEx75) were examined, the fusion protein, as judged by fluorescence of GFP, was observed tightly localized to the nuclei of most cells. SUR-5 appeared to be expressed in the VPCs. Cell types that express this fusion protein include neurons, hypodermis, Pn.p cells, body muscles, many cells of the pharynx, and a few cells of the somatic gonads. Cells that do not display the fluorescence include B, F, K , K.a, K.p, hyp3, the germ line, and the excretory duct cells. In nonmosaic animals, the intensity varies among the cells. The intestinal cells and excretory cells are almost always very bright, whereas neurons are almost always fainter. Uterine cells and many of the cells derived from the M cell are very faint and often difficult to see. The SUR-5GFP fusion proteins are expressed in all stages of C. elegans development. The earliest expression is at the 100- to 150-cell embryonic stage, and the fusion proteins are expressed throughout development from that stage on. The same expression pattern is seen when this array is integrated into one of the chromosomes. pTG96_1 is still localized in the nuclei of most cells. The expression pattern is the same as that seen from the array containing pTG96 (with NLS), but the nuclear localization is not as tight, and there appears to be some diffusion of SUR-5GFP proteins from the nucleus to the cytoplasm. Primary Identifier  Expr1416
Remark  Integrated transgenic line not described in the article. Subcellular Localization  Both constructs are expressed in nuclei; and a relatively small amount of SUR-5GFP fusion protein from pTG96_1 is detected in cytoplasm.
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29 Anatomy Terms

Definition Name Synonym Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion. body wall musculature body muscle WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
Major cell type of nervous tissue, specialized for transmission of information in the form of patterns of impulses. neuron neurone WBbt:0003679
H-shaped cell associated with the excretory system, largest cell in C. elegans. excretory cell excretory canal cell WBbt:0005812
entire syncytium of hyp7 hyp7 syncytium hyp7 WBbt:0005734
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite. P5.p hermaphrodite   WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite. P4.p hermaphrodite   WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite. P3.p hermaphrodite   WBbt:0008112
  P8.p hermaphrodite   WBbt:0008133
  P7.p hermaphrodite   WBbt:0008129
  P6.p hermaphrodite   WBbt:0008125
Posterior descendent cell of P1 fuses into postembryonic large hypodermal syncytium hyp7. P1.p   WBbt:0006889
Tail ventral hypodermis hyp10 lineage name: ABplppppppp WBbt:0004378
descendent cell of P11, posterior P11.p   WBbt:0006899
descendent cell of P10, posterior P10.p   WBbt:0006898
Posterior descendent cell of P9 fuses into postembryonic large hypodermal syncytium hyp7. P9.p   WBbt:0006897
Tail ventral hypodermis, 8th hypodermis cell hyp8   WBbt:0004381
Posterior descendent cell of P2 fuses into postembryonic large hypodermal syncytium hyp7. P2.p   WBbt:0006890
Tail ventral hypodermis, 9th hypodermis cell hyp9   WBbt:0004380
Cylindrical hypodermal syncytium in head hyp2 lineage name: ABalpaapppp WBbt:0004692
Tail dorsal hypodermis hyp11 lineage name: Cpappv WBbt:0004377
Cylindrical hypodermal syncytium in head hyp5 lineage name: ABarpappap WBbt:0004685
descendent cell of P12, posterior P12.p   WBbt:0006900
Cylindrical hypodermal syncytium in head hyp4 lineage name: ABarpapapa WBbt:0004687
Cylindrical hypodermal syncytium in head hyp1 lineage name: ABalpaapppa WBbt:0004694
organ producing either sperm or ova. gonad   WBbt:0005175
Preanal hypodermis hyp12 lineage name: P12.pa WBbt:0004376
Cylindrical hypodermal syncytium in head hyp6 lineage name: ABarpaapaa WBbt:0004679
toroidal epithelial cell that make up the bulk of the uterus, four cells anterior and four cells posterior to the vulva. uterine toroidal epithelial cell ut cell WBbt:0006784

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00006351 sur-5 K03A1.5 Caenorhabditis elegans

1 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  All C. elegans development stages, including embryo, larva and adult stage. all stages Ce WBls:0000002