WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Log in

Expression Pattern :

Pattern	RME-8 expression in other cell types was examined by whole mount immunostaining. RME-8 is expressed in almost all cells. RME-8 was highly expressed in the lateral hypodermis, muscle, and the developing germ cells in larvae. RME-8 staining was extremely abundant in the developing uterus of L4 worms. RME-8 expression was examined in dissected adult ovaries by immunofluorescence. RME-8 was highly expressed in oocytes.	Primary Identifier	Expr2399
Remark	Similar expression patterns and subcellular localizations were observed by both antibody staining and RME-8::GFP reporter expression, except that RME-8::GFP was not expressed in the germline, most likely because of a general silencing of transgenes in the C. elegans germline. These observations suggest that this RME-8::GFP fusion is an accurate reporter of endogenous RME-8 expression and subcellular localization.	Subcellular Localization	Immunofluorescence: RME-8 staining was concentrated in the cortex of oocytes beneath the plasma membrane. At high magnification, RME-8 staining was localized to punctate structures that were 0.20.4 um in diameter. RME-8 staining in the rme-8(b1023) missense mutants was diminished and mostly diffuse in the oocyte cytoplasm with little localization to cortical punctate structures . Similar mislocalization was also observed in other cell types in the mutant worms. Similar expression patterns of RME-8 in somatic cells were observed with the RME-8::GFP reporter. In most cells, RME-8::GFP-labeled small puncta with sizes similar to the puncta stained with RME-8 antibodies. In live coelomocytes, RME-8::GFP labeled the limiting membranes of larger vesicles, with diameters ranging from 0.5 to 4.0 um. By serial thin-section confocal microscopy, these vesicles appeared to be round regardless of their sizes, but after fixation, these RME-8::GFP-labeled vesicles appeared smaller, as if they had collapsed. Whole mount immunostaining: in all the cells examined, RME-8 staining was localized to cytoplasmic punctate structures similar to those seen in the oocytes.

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
Contractile cell that makes up muscle tissue.	muscle cell		WBbt:0003675
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004378	rme-8	F18C12.2	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038

Questions? Comments? Click here!